IJSRSET151452 | Received: 11 August 2015 | Accepted: 16 August 2015 | July-August 2015 [(1)4: 283-285] © 2015 IJSRSET | Volume 1 | Issue 4 | Print ISSN : 2395-1990 | Online ISSN : 2394-4099 Themed Section: Engineering and Technology 283 A Review on Use of Chromatography for Analytical Applications Sunil J. Kulkarni * 1 , Ajaygiri K. Goswami 2 , Nilesh L. Shinde 3 * 1,3 Datta Meghe College of Engineering, Airoli, Navi Mumbai, Maharashtra, India. 2 University Institute of Chemical Technology, North Maharashtra University, Jalgaon, Maharashtra ABSTRACT Analysis of the sample is very important in the research and development field. In pharmaceutical and fine chemicals industries the presence of very minute amount of unwanted material can cause very significant effect on quality. In research, the determination of heavy metal, phenol, dye and many other components in the sample of water, raw material or product is key to accuracy of the results and its interpretation. Spectroscopy is handy for detection of components. For very small amount it is not adequate. Chromatography and its various forms are very useful for analysis. The present review summarizes the research and studies on use of chromatography for analysis. Keywords: Retention Factor, Stationary Phase, Mobile Phase, Solvents. I. INTRODUCTION Analysis of the sample is very important aspect of chemical and environmental engineering. The analysis of the wastewater for determination of minute impurities is important from health point of view. The presence of dyes, phenol, organic matter and heavy metals is harmful [1, 2, 3]. It is very important to analyse the water in order to select proper treatment technology. The determination chemical oxygen demand (COD) is normally done by using COD digestion apparatus and potassium dichromate as an oxidizing agent against Mohr’s salt [4]. The phenol determination can be done by titration. For more accurate results, spectrophotometric methods are useful. For determination of heavy metals atomic absorption spectrophotometers is useful [5]. The energy dispersed x ray diffraction is also used for determination of various components in solid and liquid samples [6]. The analysis of dyes is generally done by spectrophotometric method [7]. Various chemical analysis methods can be used for glucose and starch [8, 9, 10]. Chromatography is very useful and important analytical technique. It is a method used for separating organic and inorganic compounds. It can be used to find out composition of solids and liquids. It can also be used to find unknown material in the sample. This aspect is important in forensic chemistry. In chromatography two phases, stationary and mobile, are defined. The retention factor (R f ) is indication of how far compound travels in particular solvent. The chromatography finds wide application in research and development. The present review summarizes the studies and investigations on various aspects of chromatography in the analytical applications. II. STUDIES AND RESEARCH ON CHROMATOGRAPHY FOR ANALYTICAL APPLICATIONS Kupiec discussed high-performance liquid chromatography as analytical method [11]. He discussed various aspects of chromatographic separation. He explained retention, resolution and sensitivity. He discussed the mechanisms of chromatography including adsorption, partition, ion-exchange and size an exclusion. He concluded that HPLC is most widely used analytical method in pharmaceutical industries and laboratories. Curry discussed application of chromatography in forensic science [12]. It is difficult for forensic analyst to get high enough purity to obtain such data as % carbon, % hydrogen, % nitrogen, mixed melting points. Usually they do not get enough data required for the analysis. According to him paper chromatography provides a valuable addition to his armoury. Paper chromatography offers separation of similar material on microgram scale.