49 PRINT ISSN: 2394-6679 | E-ISSN: 2347-7881 Vol. 3, Issue 6 | magazine.pharmatutor.org Zero Order and Area under Curve Spectrophotometric Methods for Determination of Fluoxetine Hydrochloride in Pharmaceutical Formulation Mali Audumbar*, Jadhav Santosh, Hake Gorakhnath, Tamboli Ashpak Department of Pharmaceutics, Sahyadri College of Pharmacy, Methwade, Sangola, Solapur, Maharashtra, India. *maliaudu442@gmail.com ABSTRACT Simple, fast and reliable spectrophotometric methods were developed for determination of Fluoxetine Hydrochloride in bulk and pharmaceutical dosage forms. The solutions of standard and the sample were prepared in Distilled Water. The quantitative determination of the drug was carried out using the zero order derivative values measured at 226 nm and the area under the curve method values measured at 220-231 nm (n=2). Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of Fluoxetine Hydrochloride using 5-25ʅg/ml (r²=0.999 and r²=0.997) for zero order and area under the curve spectrophotometric method. All the proposed methods have been extensively validated as per ICH guidelines. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. Developed spectrophotometric methods in this study are simple, accurate, precise and sensitive to assay of Fluoxetine Hydrochloride in tablets. Keywords: Fluoxetine Hydrochloride, UV visible spectrophotometry, AUC, Method Validation INTRODUCTION Fluoxetine hydrochloride, (±)- N-methyl-3-Phenyl-3- ΀;α,α,α-trifluoro- p -tolyl)] propylamine hydrochloride ( Figure 1), is an antidepressant drug used for the handling of unipolar mental depression. Fluoxetine (FLX) is the most widely prescribed selective serotonin reuptake inhibitor antidepressant drug [1,2] . FXT has been shown to have comparable efficacy to tricyclic antidepressants but with fewer cardiovascular and anticholinergic side effects. It is also effective in treatment of the obsessive compulsive disorders. Fluoxetine is extensively metabolized by N-demethylation in liver into its active metabolite norfluoxetine. Fluoxetine hydrochloride is also used in a variety of disorders in addition to depression [3,4,5] . Beneficial responses have been reported in obsessive compulsive disorders, pain syndromes including diabetic neuropathy and fibrositis, panic disorders and nervous bulimia (American Hospital Formulary Service, Drug Information 93) [6,7] .Literature survey revealed several analytical methods UV spectrophotometry [8,9] and HPLC [10] have been reported in bulk, pharmaceutical dosage form for determination of Fluoxetine Hydrochloride. To our notice, so far no UV- spectrophotometric method using Zero Order and Area under Curve (AUC) has been reported for the determination of Fluoxetine Hydrochloride in bulk and tablets. Hence an attempt has been made to develop new Zero Order and Area under Curve Spectrophotometric methods for estimation of Fluoxetine Hydrochloride in bulk and pharmaceutical formulations with good accuracy simplicity, precision and economy. Molecular formula: C 17 H 18 F 3 NO Molecular weight: 309.32613 g/mol How to cite this article: A Mali, S Jadhav, G Hake, A Tamboli; Zero Order and Area under Curve Spectrophotometric Methods for Determination of Fluoxetine Hydrochloride in Pharmaceutical Formulation; PharmaTutor; 2015; 3(6); 49-54