www.wjpr.net Vol 4, Issue 10, 2015. 242 Hasan et al. World Journal of Pharmaceutical Research ESTIMATION OF OXALATE CONTENTS IN MACROTYLOMA UNIFLORUM (LAM.) VERDC., PHASEOLUS LUNATUS LINN., AND PHASEOLUS VULGARIS LINN. Salman Ahmed 1 , Muhammad Mohtasheemul Hasan 1* and Zafar Alam Mahmood 2 1 Department of Pharmacognosy, Faculty of Pharmacy, University of Karachi, Karachi-75270, Pakistan. 2 Colorcon Limited – UK, Flagship House, Victory Way, Crossways, Dartford, Kent, DA2 6 QD- England. ABSTRACT The seed flours of Macrotyloma uniflorum (Lam.) Verdc., Phaseolus lunatus Linn., and Phaseolus vulgaris Linn., were investigated for their total, soluble and insoluble oxalate contents by HPLC. The total oxalate content of Macrotyloma uniflorum, Phaseolus lunatus and Phaseolus vulgaris were found to be 1.24, 1.77 and 1.71 mg/ g respectively. The % age of soluble oxalate were Macrotyloma uniflorum (19.50), Phaseolus lunatus (15.08) and Phaseolus vulgaris (15.88). The calculated soluble oxalate: calcium ratio of all tested legume flours are lesser than one and hence will not create any resistance in calcium bioavailability. KEYWORDS: Macrotyloma uniflorum (Lam.) Verdc., Phaseolus lunatus Linn., Phaseolus vulgaris Linn., oxalate. INTRODUCTION Oxalate is widely distributed in plant foods as potassium, sodium and ammonium oxalates (water-soluble form) and as insoluble calcium oxalates. Oxalate forms strong chelates with dietary calcium, thus rendering the complex unavailable for absorption and assimilation. It precipitates as insoluble salts accumulating in the renal glomeruli and contributes to the development of renal disorders. [1] Urinary oxalate originates from a combination of absorbed dietary oxalate and endogenous formation from oxalate precursors such as ascorbic acid and glyoxylate. [2] While other factors have to be considered in the development of renal disorder. World Journal of Pharmaceutical Research SJIF Impact Factor 5.990 Volume 4, Issue 10, 242-249. Research Article ISSN 2277– 7105 Article Received on 8 Aug 2015, Revised on 28 Aug 2015, Accepted on 17 Sep 2015 *Correspondence for Author Prof. Muhammad Mohtasheemul Hasan Department of Pharmacognosy, Faculty of Pharmacy, University of Karachi, Karachi- 75270, Pakistan.