www.wjpps.com Vol 4, Issue 08, 2015. 1238 Kannahi et al. World Journal of Pharmacy and Pharmaceutical Sciences PHOSPHATE SOLUBILIZING POTENTIALITY OF BACILLUS SUBTILIS AND PSEUDOMONAS AERUGINOSA ON VIGNA UNGUICULATA GROWTH USING AGROWASTE AS A SUBSTRATE M. Kannahi* and R. Megala P.G and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College, Mannargudi, Tamil Nadu, India. ABSTRACT In the present study, phosphate solubilizing microorganisms were isolated from the rhizosphere soil. The phosphate solubilizing microorganisms such as Bacillus subtilis and Pseudomonas aeruginosa were identified based on cultural, morphological and biochemical characteristics. The agrowaste such as Rice Bran (RB), Wheat Bran (WB), Black Gram Husk (BGH) and Farm Yard Manure (FYM) were decomposed by the treatment of bacterial strains namely, Bacillus subtilis and Pseudomonas aeruginosa for phosphate solubilizing activity. Two set of treatments were carried out and analysed. The first set of treatment was performed with partially decomposed agrowaste, phosphate solubilizing bacteria and sowing with seeds. The second set of treatment was performed with raw agrowaste and phosphate solubilizing bacteria. After 15 days of decomposition, the seeds were sown in the second set of pots. In both set of treatment, the morphometric and phytochemical parameters were analysed at different intervals (30 th , 45 th and 60 th days). KEYWORDS: Agrowaste, Decomposition, Phosphate Solubilizing Bacteria, Biochemical test, Vigna unguiculata. INTRODUCTION Bio-fertilizers are one of the best modern tools for agriculture. It is a gift of our modern agricultural science. Biofertilizers are applied in the agricultural field as a replacement to our WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES SJIF Impact Factor 5.210 Volume 4, Issue 08, 1238-1244. Research Article ISSN 2278 – 4357 Article Received on 30 May 2015, Revised on 25 June 2015, Accepted on 18 July 2015 *Correspondence for Author M. Kannahi P.G and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College, Mannargudi, Tamil Nadu, India.