are blocked by ablation of miR210 expression during senescence by specific AntagomiRs. Our data shed new light on the ability of senescent stroma to affect prostate cancer malignancy, sustaining the development of a favorable microenvironment for cancer progression, recruiting/ organizing vessels and sustaining inflammation. SW03.S13–72 The effect of combined heavy metal ions on copA, nikA and czcD genes expression of metal-resistant bacilli A. Margaryan 1,2 , N.-K. Birkeland 2 , H. Panosyan 1 , Y. Popov 1 and A. Trchounian 1 1 Department of Microbiology, Plants and Microbes Biotechnology, Yerevan State University, Yerevan, Armenia, 2 Department of Biology, University of Bergen, Bergen, Norway Heavy metal resistant microorganisms, thriving in environments with excessive concentrations of heavy metals, have evolved a variety of adaptation strategies. Reduced accumulation based on an active efflux of the bivalent cations is the primary resistance mechanism developed in prokaryotes. The goal of the present work was to investigate the expression of the copA, nikA and czcD genes of metal-resistant bacilli as a metal stress response to the presence of different concentrations of a combination of Cu (II), Ni(II), Cd(II) and Zn(II) metal ions. The test organisms were Bacillus subtilis AG4, Bacillus megaterium AA1, Geobacillus pulidus AA4 and Clostridium saccharolyticum LA5 strains isolated from different territories in Armenia with elevated levels of toxic metals. The resistance patterns, expressed as minimum inhibitory concentrations, were analyzed by using the agar dilution method. All isolates showed high resistance to Cu(II) and Ni(II) (up to 5 mM), but were more sensitive to Cd(II) and Zn(II) (up to 0.4 and 1 mM, respectively). PCR combined with DNA sequence analysis was used to investigate the presence of the copA, nikA and czcD genes, which are often responsible for resistance to mentioned ions. The presence of copA and nikA genes was con- firmed in all the strains. czcD was confirmed only in B. subtilis AG4 and C. saccharolyticum LA5. Transcription analysis of selected genes in the presence of different concentrations of metal ions in the growth medium was performed by RT-qPCR. Expres- sion of the czcD gene was not detected. Highest expression copA and nikA genes was observed in the presence of a combination of Cu(II), Ni(II), Cd(II) and Zn(II) in 0.016, 0.017, 0.01 and 0.015 mM concentrations, correspondingly, i.e. at very low metal concentrations. The absence or low expression of copA and nikA genes at high metal concentrations indicates that alternative resis- tance mechanisms might be present. The work was supported by the CPEA-2011/10081 grant from the Norwegian Cooperation Programme in Higher Education with Eurasia. SW03.S13–73 Protective effect of natural polyphenol complex of red wine under radioinduced oxidative-nitrative stress N. Sybirna, M. Sabadashka, U. Staranko, V. Drel and L. Datsyuk Ivan Franko Lviv National University, Lviv, Ukraine Overproduction of reactive Oxygen (ROS) and Nitrogen (RNS) species play a key role in the development of pathological changes in the organism after exposure to low doses of ionizing radiation. Radioinduced changes in the prooxidant-antioxidant balance are caused by disorders in NO synthesis and metabolism and by the unbalanced function of antioxidant enzymes. It is known, that polyphenolic compounds of red wine exhibit pronounced antioxidant properties under development of different diseases. Therefore studing their effect on the pathological changes in the organism under low doses of radiation presents a high inter- est. Under the X-ray exposure (30 cGy) was observed increasing in two times the content of thiobarbituric acid reactive substances (TBARS) and nitrotyrosine-modified (NT) proteins in the cortical layer of rat kidney. At the same time, the activities of superoxide dismutase (SOD) and NO-synthase (NOS) decreased on 72 h post- irradiation, while at the earlier time points (24 and 48 h after irra- diation) the activities of SOD, catalase (CT) and glutathione perox- idase (GPO) increased when compared to control. Consumption of preparation of natural polyphenolic complex of wine (NPCW) for 10 days before and during 3 days after irradiation caused an increase in antioxidant capacity of the cortical layer of rat kidney. The polyphenols reduced the content of TBARS in rat kidney after irradiation by 1.4 times compared to irradiated rats without treat- ment, while the activity of antioxidant enzymes and NOS as well as the content of NO stable products did not differ from controls. The consumption of NPCW also decreased the content of NT pro- teins, a key marker of nitrative stress, in the cortical layer of rat kidney compared to irradiated nontreated rats. Thereby, our results show that the preparation of NPCW diminishes oxidative-nitrative stress caused by the action of low doses of ionizing radiation. Red wine polyphenols implement their radioprotective function through ROS and RNS scavenging. In this way the activities of antioxidant defense system enzymes and L-arginine/NO system components were changed and cor- rected after irradiation. SW03.S13–74 14-3-3 positively regulates murine protein serine-threonine kinase 38 in a phosphorylation-dependent manner H. Ha, R. Manoharan and H.-A. Seong Department of Biochemistry, School of Life Sciences, Chungbuk National University, Cheongju, Korea Murine protein serine-threonine kinase 38 (MPK38)/maternal embryonic leucine zipper kinase (MELK) is a member of the family of AMP-activated protein kinase (AMPK) that respond to changes in cellular energy state. In this study, we demonstrate that 14-3-3 has a positive role in the regulation of MPK38 activ- ity and function. The physical association of MPK38 and 14-3-3 is mediated through their C-terminal domains. MPK38 directly interacts with and phosphorylates 14-3-3 (Ser 156 of 14-3-3 theta and Ser 158 of 14-3-3 sigma). In addition, ectopic expression of wild-type 14-3-3, but not the 14-3-3 theta S156A (or 14-3-3 sigma S158A) mutant, dose-dependently enhances MPK38- dependent ASK1, TGF-beta, and p53 signaling by stabilizing the MPK38 protein, suggesting that 14-3-3 phosphorylation by MPK38 contributes to the stimulation of MPK38 activity and function. We also demonstrate an in vivo role of 14-3-3 in the regulation of MPK38 function using 14-3-3-null HCT116 cells. Together, our present results provide evidence that 14-3-3 func- tions as a physiological activator of MPK38 in cells. FEBS Journal 280 (Suppl. 1) (2013) 3–617 ª 2013 The Authors. FEBS Journal ª 2013 FEBS 231 SW03 Mechanisms of Communication and Signaling Abstracts