Research Article Application of FTIR-ATR Spectroscopy to Determine the Extent of Lipid Peroxidation in Plasma during Haemodialysis Adam Oleszko, 1,2 Sylwia OlsztyNska-Janus, 1 Tomasz Walski, 1,2 Karolina Grzeszczuk-KuT, 1,2 Jolanta Bujok, 2 Katarzyna GaBecka, 1,2 Albert Czerski, 2,3 Wojciech Witkiewicz, 2 and MaBgorzata Komorowska 1,2 1 Institute of Biomedical Engineering and Instrumentation, Faculty of Fundamental Problems of Technology, Wrocław University of Technology, Wybrze˙ ze Wyspia´ nskiego 27, 50-370 Wrocław, Poland 2 Regional Specialist Hospital in Wrocław, Research and Development Centre, Kamie´ nskiego 73a, 51-124 Wrocław, Poland 3 Department of Animal Physiology and Biostructure, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Norwida 31, 50-375 Wrocław, Poland Correspondence should be addressed to Małgorzata Komorowska; malgorzata.komorowska@pwr.edu.pl Received 24 November 2014; Accepted 29 January 2015 Academic Editor: Silvia Maria Doglia Copyright © 2015 Adam Oleszko et al. his is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. During a haemodialysis (HD), because of the contact of blood with the surface of the dialyser, the immune system becomes activated and reactive oxygen species (ROS) are released into plasma. Particularly exposed to the ROS are lipids and proteins contained in plasma, which undergo peroxidation. he main breakdown product of oxidized lipids is the malondialdehyde (MDA). A common method for measuring the concentration of MDA is a thiobarbituric acid reactive substances (TBARS) method. Despite the formation of MDA in plasma during HD, its concentration decreases because it is removed from the blood in the dialyser. herefore, this research proposes the Fourier Transform Infrared Attenuated Total Relectance (FTIR-ATR) spectroscopy, which enables determination of primary peroxidation products. We examined the inluence of the amount of hydrogen peroxide added to lipid suspension that was earlier extracted from plasma specimen on lipid peroxidation with use of TBARS and FTIR-ATR methods. Linear correlation between these methods was shown. he proposed method was efective during the evaluation of changes in the extent of lipid peroxidation in plasma during a haemodialysis in sheep. A measurement using the FTIR-ATR showed an increase in plasma lipid peroxidation ater 15 and 240 minutes of treatment, while the TBARS concentration was respectively lower. 1. Introduction Dialysis therapy allows for the removal of metabolic waste products and excess water and electrolytes from the blood- stream of patients with renal failure. he treatment is per- formed by putting the patient’s blood through the dialyser where it comes into contact with a semipermeable membrane with dialysis luid, the content of which allows for regenera- tion of the blood bufer system. Contact of the patient’s blood with surfaces of drains and the dialyser leads to activation of the immune system [1], resulting in intensiied production of reactive oxygen species (ROS) via the activation of neutrophils and platelets— a so-called “oxidative burst” [2]. Apart from the super oxide radical, one ROS whose concentration rises during an oxidative burst is hydrogen peroxide. he ROS present in the blood causes oxidation of the compounds contained therein. Plasma lipids and phospholipids, being part of the erythrocyte cell membrane, are particularly susceptible to ROS activity [1]. Research conducted by Haklar et al. [3] conirms the pres- ence of a lipid peroxidation process during haemodialysis. Ater the treatment, an increase in the quantity of conjugated dienes from 3.75 to 5.31 nmol per 1 mol of lipids extracted from plasma was observed. It has also been proven that, during haemodialysis, a peroxidation of cholesterol from the cell membranes of erythrocytes [4] and a decrease in the concentration of antioxidants in plasma [5] take place. Hindawi Publishing Corporation BioMed Research International Volume 2015, Article ID 245607, 8 pages http://dx.doi.org/10.1155/2015/245607