Protein Sorting at the trans-Golgi Network Yusong Guo, Daniel W. Sirkis, and Randy Schekman Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, California 94720-3200; email: schekman@berkeley.edu Annu. Rev. Cell Dev. Biol. 2014. 30:169–206 First published online as a Review in Advance on August 18, 2014 The Annual Review of Cell and Developmental Biology is online at cellbio.annualreviews.org This article’s doi: 10.1146/annurev-cellbio-100913-013012 Copyright c  2014 by Annual Reviews. All rights reserved Keywords vesicle coat proteins, Arfs, cargo adaptors, cargo receptors, phospholipids Abstract The trans-Golgi network (TGN) is an important cargo sorting station within the cell where newly synthesized proteins are packaged into distinct trans- port carriers that are targeted to various destinations. To maintain the fi- delity of protein transport, elaborate protein sorting machinery is employed to mediate sorting of specific cargo proteins into distinct transport carriers. Protein sorting requires assembly of the cytosolic sorting machinery onto the TGN membrane and capture of cargo proteins. We review the cytoso- lic and transmembrane sorting machinery that function at the TGN and describe molecular interactions and regulatory mechanisms that enable ac- curate protein sorting. In addition, we highlight the importance of TGN sorting in physiology and disease. 169 Annu. Rev. Cell Dev. Biol. 2014.30:169-206. Downloaded from www.annualreviews.org by Yeshiva University - Albert Einstein College of Medicine on 11/14/14. For personal use only.