Chapter 4 Adolescent nicotine exposure transiently increases high-ainity nicotinic receptor expression in the rat medial prefrontal cortex. Danielle S Counotte 1 , Milena Moretti 4 , Marek T Smoluch 2 , Hubertus Irth 2 , Francesco Clementi 4 , Francois Hogenboom 3 , Anton NM Schoffelmeer 3 , August B Smit 1 , Sabine Spijker 1 *, Cecilia Gotti 4 * Departments of 1 Molecular and Cellular Neurobiology, Center for Neurogenom- ics & Cognitive Research, Neuroscience Campus Amsterdam, VU University, The Netherlands 2 Analytical Chemistry & Applied Spectroscopy, VU University, The Netherlands 3 Anatomy and Neurosciences, VU Medical Center, The Netherlands, 4 Department of Medical Pharmacology, Cellular and Molecular Pharmacology, CNR, Institute of Neuroscience, University of Milan, Milan, Italy. *Authors contributed equally Manuscript in preparation 83