_____________________________________________________________________________________________________ *Corresponding author: E-mail: fahimullah320@yahoo.com; Journal of Advances in Biology & Biotechnology 3(2): 84-89, 2015; Article no.JABB.2015.035 ISSN: 2394-1081 SCIENCEDOMAIN international www.sciencedomain.org Prevalence and Molecular Detection of Giardia Spp in Different Drinking Water Sources in Karak Lubna Hassan 1 and Fahim Ullah 2* 1 Department of Zoology, Kohat University of Science and Technology (KUST), 26000, Khyber, Pukhtunkhwa, Pakistan. 2 Department of Environmental Engineering, College of Environment, Hohai University, 1 Xikang Road, Nanjing, 210098, P.R. China. Authors’ contributions This work was carried out in collaboration between both authors. Author LH collected the samples and perform molecular detection. Author FU designed the study and performed analysis and wrote the first draft of the manuscript and also managed the analyses of the study. Both authors read and approved the final manuscript. Article Information DOI: 10.9734/JABB/2015/15937 Editor(s): (1) Joana Chiang, Department of Medical Laboratory Science and Biotechnology, China Medical University, Taiwan. (2) Rafael A. Cañas, Department of Molecular Biology and Biochemistry, Málaga University, Spain. Reviewers: (1) Arturo Solís Herrera, Human Photosynthesis Research Center, R & D & I, México. (2) Anonymous, USA. (3) Wahome Caroline Nyawira, Plant and Microbial Sciences Department, Kenyatta University, Kenya. (4) Anonymous, Argentina. Complete Peer review History: http://www.sciencedomain.org/review-history.php?iid=929&id=39&aid=8780 Received 26 th December 2014 Accepted 9 th March 2015 Published 13 th April 2015 ABSTRACT Aims: The study was conducted to know the prevalence of Giardia spp in different sources of water in District Karak Khyber Pukhtunkhwa, Pakistan. Methods of Study: A total of 65 water samples was collected from different villages of District Karak was tested in the lab of the Department of Zoology in Kohat University of Science & Technology with the method of using PCR (Polymerase chain reaction) to detect the prevalence of G in tube well, bore, drainage and tap water. Results: From the result the prevalence of G in tube well, drainage and bore water were found 10.76%, 1.53% and 1.53% respectively while in the sample of tap water there will be no prevalence of G was detected. Conclusion: From the result it was concluded that a proper treatment of water for human consumptions is required. Original Research Article