43-3-12 Journal of Microwave Power & Electromagnetic Energy ONLINE Vol. 43, No. 3, 2009 Diana Martin 1 , Sabin Cinca 2 , Irina Margaritescu 3 , Monica Neagu 4 , Nicusor Iacob 1 , Daniel Ighigeanu 1 , Constantin Matei 1 , Gabriela Craciun 1 , Elena Manaila 1 , Doru Aurel Chirita 3 and Mihaela Moisescu 5 1 National Institute for Lasers, Plasma and Radiation Physics, Bucharest, Romania * diana.martin@inflpr.ro 2 Oncology Institute “A. Trestioreanu”, Bucharest, Romania 3 Military Clinical Hospital “Carol Davila”,Bucharest, Romania 4 National Institute “Victor Babes”, Bucharest, Romania 5 University of Human Medicine and Pharmacy ”Carol Davila”, Bucharest,Romania The paper presents two radiation exposure facilities (REFs) which permit separate and simul- taneous irradiation with microwaves (MW) of 2.45 GHz and electron beams (EB) of 6.23 MeV for malignant melanoma (MM) cell investigations, in vitro (MW+EB-REF-vitro) and in vivo (MW+EB- REF-vivo). The REFs are specifically designed for the following medical studies: 1) The effects of separate and combined (successive and simultaneous) MW and EB irradiation on the B16F10 mouse - MM cell cultures without/with drugs incubation; 2) The effects of separate and combined MW and EB irradiation on human blood components irradiated in samples of integral blood from healthy donors and from donors with MM; 3) The effects of separate and combined MW and EB whole body irradiation on the C57 BL/6 mice bearing MM without/with drugs administration. Sev- eral representative results obtained by experiments with REFs in vitro and in vivo are discussed. The most important conclusion of the experimental results is that low dose-total body MW+EB irradiation combined with drugs administration could present a valuable potential for an advanced study in malignant melanoma therapy. Submission Date: 18 August 2008 Acceptance Date: 15 May 2009 Publication Date: 28 July 2009 CoMbINED MICrowAvE AND ElECtroN bEAM ExPoSurE FACIlItIES For MEDICAl StuDIES AND APPlICAtIoNS Keywords: microwave, electron beam, combined exposure, B16F10 cell, C57 BL/6 mouse INTRODUCTION In addition to routine conventional radiotherapy techniques [Olofsson, 2005], electron beams (EB) are presently used or are under study for cancer specialized therapies such as intensity modulated radiation therapy and total body electron irradiation [Leung, 1998, Shouman, 2004, Margaretic, 2002, Van Dyk, 1986]; irradiation of blood and blood components [Moroff, 1997, Buston, 2000]; virus inactivation and vaccine preparation [Smolko, 2005], and others. Microwaves (MW) are presently used or are under study for therapeutic applications in areas such as cardiology, urology, surgery, ophthalmology, cancer therapy, and for diagnostic applications in areas such as cancer detection, organ imaging, and more [Rosen, Guest Editor: Dr. Satoshi Horikoshi, Tokyo University of Science, Chiba, Japan