Abstracts / Toxicology Letters 229S (2014) S40–S252 S43 Purpose: A range of different in vitro chemistry-based (DPRA, GSH reactivity) and cell-based methods (MUSST, hCLAT, Kerati- nosens) have been developed and some of them are currently evaluated for their applicability to cosmetic ingredients and their physicochemical diversity. Although these assays appear to be promising for hazard identification, potency assessment is still lim- ited. Possible limitations may be linked to the metabolism that may differ between the models and native skin, to the bioavailability which is not considered in monolayer cultures, and to the danger signal that may be different in monolayers as compared to a natural tridimensional microenvironment. Method: We have developed SENS-IS, a method, based on the quantitative analysis of specific biomarkers expressed in 3D recon- structed epidermis (Episkin ® ), thus providing a possible way to encompass these limitations and come closer to potency assess- ment. The aim of this study was to confirm the transferability and reproducibility of the SENS-IS protocol and its ability to correctly classified sensitizing potency in 5 classes similarly to the LLNA. Results and conclusions: Three laboratories participated in this SENS-IS ring study using 19 chemicals tested blindly. All chemicals were similarly classified by the 3 laboratories with exception using HCA that was overestimated by one laboratory. Analysis of predic- tive capacity (with 5 class) was 84% as compare to LLNA and 100% with one class difference. These results shows that the SENS-IS assay can be steadily transferred and provides reproducible results on safety assessment of chemical products. http://dx.doi.org/10.1016/j.toxlet.2014.06.189 P-1.11 Caffeic acid phenethylester prolongs the lifespan of Caenorhabditis elegans dependent on the transcription factor DAF-16, but not SKN-1 Susannah Havermann * , Wim Wätjen Martin-Luther-University Halle-Wittenberg, Halle, Germany Oxidative stress has been linked to a variety of diseases and the ageing process. Distinct secondary plant compounds like hydroxy- cinnamic acid derivatives possess a high antioxidative capacity and have been epidemiologically connected with beneficial effects on human health. Next to direct radical scavenging, the ability of com- pounds to modulate cellular pathways has been shown to be of importance. We have compared the effects of caffeic acid phenethylester (CAPE) on redox-sensitive pathways in cell culture-based assays and the model organism Caenorhabditis elegans. CAPE decreased ROS accumulation in Hct116 human colon carcinoma cells and the nematode after application of stress (DCF assay). In addition to these antioxidative effects, CAPE prolonged the mean life span by 9%. This effect is independent from the transcription factor SKN-1 since the localisation is not modulated by CAPE as analysed using a SKN-1::GFP expressing strain. In accordance, a knock-down of SKN-1 did not alter the effect of CAPE on the lifespan. Instead, the modulation of a different central ageing-related pathway was detected using a DAF-16::GFP (FoxO homologue) expressing strain. A direct dependence of CAPE-mediated life prolongation on DAF- 16 was shown by lifespan analysis with DAF-16 mutated C. elegans. In Hct116 cells CAPE shows a different mode of action: We were able to show that CAPE is a potent activator of Nrf2 but does not activate FoxO4. Conclusion: CAPE modulates distinct biological functions depending on the model system used. http://dx.doi.org/10.1016/j.toxlet.2014.06.190 P-1.12 Perinatal exposure to low dose glufosinate ammonium induces autism-like phenotypes in mice Anthony Laugeray 1 , Ameziane Herzine 1,2,* , Olivier Perche 1,3 , Olivier Richard 1,2 , Arnaud Menuet 1,2 , Séverine Mazaud-Guittot 4 , Laurianne Lesne 4 , Sylvain Briault 1,3 , Bernard Jegou 4 , Jacques Pichon 1,2 , Céline Montecot-Dubourg 1,2 , Stéphane Mortaud 1,2 1 Immunologie et Neurogénétique Expérimentales et Moléculaires, UMR7355 CNRS, Orleans, France, 2 Université d’Orléans, Orleans, France, 3 Département de génétique, Centre Hospitalier Régionale, Orleans, France, 4 IRSET Inserm U.1085, Université de Rennes I, Rennes, France Glufosinate ammonium is one of the most widely used herbi- cides in agriculture. As is the case for most pesticides, potential adverse effects of GLA have not been studied from the perspective of developmental neurotoxicity. Early pesticides exposure may weaken the basic structure of the developing brain and cause permanent changes leading to a wide range of lifelong effects on health and/or behavior. Here we addressed the developmental impact of GLA by exposing female mice to low dose GLA during both pre- and postnatal periods and analyzed potential develop- mental and behavioral changes of the offspring during infancy and adulthood. A neurobehavioral test battery revealed significant effects of GLA on early reflex development, pup communication, affiliative behaviors, and preference for social olfactory cues, but emotional reactivity and emotional memory remained unaltered. These behavioral alterations showed a striking resemblance to changes seen in animal models of Autistic Spectrum Disorders (ASD). We observed brain weight abnormalities and alterations in neuroblast migration within the subventricular zone (Cf. poster Herzine et al. in the present meeting). We identified disturbances in the expression patterns of two genes implicated in autism-like deficits. Our work thus provides new data on the link between perinatal exposure to the herbicide GLA and the onset of autism- like symptoms later in life. It also raises fundamental concerns about the ability of current safety testing to assess risks of pesticide exposure during critical developmental periods. Keywords: Glufosinate ammonium; Autistic Spectrum Disor- ders; Perinatal exposure neurodevelopment; Mice http://dx.doi.org/10.1016/j.toxlet.2014.06.191 P-1.13 Analysis of the validated epiderm skin corrosion test (EpiDerm-SCT) and prediction model for sub-categorization according to the UN-GHS and EU-CLP Helena Kandarova 1,2,* , Silvia Letasiova 1 , Yulia Kaluzhny 2 , Patrick Hayden 2 , Mitchell Klausner 2 1 MatTek IVLSL, Bratislava, Slovakia, 2 MatTek Corp., Ashland, MA, USA Skin corrosion refers to the production of irreversible dam- age to skin manifested as visible necrosis through epidermis and into dermis. In 2004, OECD adopted two ECVAM-validated recon- structed human skin model assays for testing skin corrosion (OECD TG 431). However, OECD TG 431 does not satisfy international labelling guidelines for transport of dangerous goods since none