Pak. J. Pharm. Sci., Vol.28, No.3, May 2015, pp.933-938 933 Various fractions of Hypericum x moserianum and Hypericum ericoides possess antiglycation, anti-lipid peroxidation, antioxidative activities and non-toxic effects in vitro Ghulam Abbas 1-2 , Muhammad Shahzad 3 *, Zeb Saddiqe 4 , M Jawad Hassan 5 , Sumbal Saba 6 , Jamal Rafique 6 , Rizwana Malik 6 and Hidayat Hussain 7 1 Department of Biochemistry, University of Health Sciences, Lahore, Pakistan 2 Department of Biological Sciences and Chemistry, University of Nizwa, Nizwa-616, Oman 3 Department of Pharmacology, University of Health Sciences, Lahore, Pakistan 4 Department of Botany, Lahore College for Women University, Lahore, Pakistan 5 ASAB, National University of Sciences and Technology (NUST), Islamabad, Pakistan 6 International Center for Chemical and Biological Sciences, HEJ RIC, University of Karachi, Karachi, Pakistan 7 UoN Chair of Oman's Medicinal Plants and Marine Natural Products, University of Nizwa, Nizwa-616, Oman Abstract: In the present study, two species Hypericum x moserianum and Hypericum ericoides which belong to genus Hypericum were evaluated for their potential antiglycation, antioxidant, anti lipid peroxidation and cytotoxic activities. These species are widely used in folk medicine and to the best of our knowledge there were no previous reports regarding antioxidant, anti-glycation and cytotoxicity studies of these species. Among the crude methanol extracts and fractions of both the species, the ethyl acetate fraction of H. x moserianum exhibited promising antioxidant activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) with IC50 129.084±1.215µg/ml, followed by methanol extract (IC50=232.083±1.215µg/ml) and aqueous fraction (IC50=266.962±2.213µg/ml). The ethyl acetate fraction of H. ericoides exhibited IC50 value of 295.088±2.320µg/ml. In antiglycation assay, the ethyl acetate fraction of H. x moserianum showed 52.096% inhibition at 500µg/ml. For lipid peroxidation assay, the dichloromethane, aqueous and n- hexane fractions of H. x moserianum showed 67.241, 66.147 and 64.213% inhibition respectively, while aqueous fraction of H. ericoides exhibited 67.404% inhibition at 500µg/ml. In cytotoxicity assay, all fractions of both the species were found to be non-toxic on mouse fibroblast 3T3 cells with IC50 value greater than 30µg/ml as compared to cycloheximide with IC50 value 0.073±0.1µg/ml used as a standard. It was concluded from the study that among the two species, crude methanolic and ethyl acetate fractions were more active regarding the antioxidant, anti-glycation activities while dichloromethane, aqueous and n-hexane fractions possessed anti-lipid peroxidation activity. Keywords: Protein Glycation, oxidative stress, antioxidants, lipid peroxidation, cytotoxicity and genus Hypericum. INTRODUCTION There is a complex link between the glycation process and oxidation during diabetes mellitus while lipid peroxidation and malondialdehyde (MDA) accumulation is also observed in diabetics. Various substances such as aminoguanidine (AG) and L-arginine have been reported to prevent the formation of advanced glycation endproducts (AGEs) and lipid peroxidation (Jakug et al., 1999). The difference in the production of reactive oxygen species and quantities of antioxidants present in the body plays an important role in multiple diseases. Various reactive species of oxygen, nitrogen and chlorine are generated in vivo through various mechanisms (Halliwell and Gutteridge, 1999). The antioxidants whether synthetic or natural origins can effectively eliminate free radicals by averting their formation, scavenging them or increasing their decomposition rate. (Heinecke, 2003; Kaur and Kapoor 2002; Cesquini et al., 2003). Antioxidants protect against glycation derived free radicals and may have therapeutic potential (Ceriello et al., 1991). Vitamin E which is a well known antioxidant blocks the protein glycation by inhibiting MDA formation thus long-term complications can be reduced by reducing oxidative stress in patients with diabetes (Soon, 2004). Due to increased understanding of the damaging effects of glycation process, it is highly desirable to manage this process effectively either by prevention or by managing the consequences of glycation, which could be useful for millions of affected people (Khalifa et al., 1999; Rahbar et al., 2000). Antioxidant and anti glycation agents obtained from natural products have great therapeutic potential. Moreover, recent study discovered that compounds with combined antioxidant and anti-glycation properties are more efficient for the management of diabetes mellitus (Duraisamy et al., 2003). The genus Hypericum (Hypericaceae) comprising of more than 450 species is well known for its use in folk medicine to treat wounds, eczema, burns, bruises, arthritis, hepatitis, herpes, and the disorders of central nervous system and alimentary tract in many parts of the *Corresponding author: e-mail: gapakland@gmail.com