International Journal of PharmTech Research CODEN (USA): IJPRIF ISSN : 0974-4304 Vol.1, No.4, pp 1589-1593, Oct-Dec 2009 HYPOGLYCEMIC EFFECTS OF FRUIT JUICE OF MURRAYA KOENIGII (L) IN ALLOXAN INDUCED DIABETIC MICE Tembhurne S. V. , Sakarkar D. M. * Department of Pharmacology, Sudhakarrao Naik Institute of Pharmacy, Pusad, Yavatmal. 445204 (M.S), India. Email: dsakarkar@rediffmail.com Phone no. +91-07233247308 ABSTRACT: Oxidative stress has been defined as a disturbance in the balance the production of reactive oxygen species (ROS) and antioxidant defence system, which can lead to tissue injury. Antioxidant level in the tissue is an important factor for sensitivity of individual tissue to oxidative stress. It has been suggested that oxidative stress can play an important role in tissue damage associated with diabeties and complications. Oxidative stress in diabeties and increased of free radicals are generated which cause injury or destruction of pancreatic beta cells which can repaired or regenerated by using potent antioxidant. Thus based on reported antioxidant phytochemical constituents in fruit of Murraya koenigii the present study was aim at to investigate hypoglycemic effect of fruit juice of Murraya koenigii in mice. Based on the results of normoglycemic and OGTT model the hypoglycemic effect in alloxan induced diabetic mice was evaluated at dose level of 2.5 and 5.0 ml/kg. The results of antidiabetic study reveled that FJMK decreases blood glucose level significantly at 10 and 15 day of administration (fasted serum glucose). From results it concludes that FJMK has hypoglycemic effect. The FJMK may act as cytoprotective in alloxan induced diabetic mice because of presence of antioxidant phytochemical so it can be attributed to the intended use of fruit juice for antidiabetic dietary supplement or as a herbal drink for diabetic and in diabetic complications. Key words: Murraya koenigii, antidiabetics, dietary supplement, herbal drink. INTRODUCTION Murraya koenigii (L.) family Rutaceae is an aromatic more or less deciduous shrub or a small tree up to 6m. in height found throughout India and is commonly known as Meethi neem and karry tree, is used traditionally as antiemetic, antidiarrhoeal, febrifuge and blood purifier. The whole plant is considered to be a tonic and stomachic. The leaves are used extensively as a flavoring agent in curries and chutneys. Almost every part of this plant has a strong characteristic odour. The people of the plains, particularly of southern India, use the leaves of this plant as a spice in different curry preparations 1, 2 . In the present study, Murraya koenigii (L) was chosen since it is one of the most widely acclaimed remedies for the treatment of diabetes. M. koenigii are used as flavorings, condiment and folk medicine for the treatment of various metabolic and infectious diseases. The leaves, bark, root and fruits are used intensively in indegenious system of medicine from ancient time, as a tonic for stomache, stimulat and creative 2, 3 . Phytochemical screening of M. koenigii leaves reveled the presence of some vitamins, carbazole alkaloid, terpenoids, phenolic compounds and mineral content such as calcium, iron, zinc and vanadium etc. in addition, carbazole alkaloid present in M. koenigii leaves were reported to have antioxidant and antidiabetic activities 4, 5, 6, 7, 8, 9, 10 . There also having several biological activities of M. koenigii leaves reported for its anti-hypercholesterolemic 11, 12 as well as its efficacy against colon carcinogensis 11 . It also reported for anti-microbial, antioxidant 5, 13, 14, 15, 16 . While there is not any scientific report found on fruit of Murraya koeinigii for it’s any biological activities including antidiabetic which has reported for its leaves 4, 5, 6 . Therefore, there was a strong interest to find the possible antidiabetic activity of fruit of Murraya koenigii. MATERIAL AND METHOD Plant: The fresh fruit of Murraya koenigii were collected in the month of August 2008 from its natural habitat at Sakoli village in Nagpur region, Maharastra, India. The plant was authenticated by Dr. N. M. Dongarwar of Botany Department; RTM Nagpur University, Nagpur India. A voucher specimen (No: 9439) was deposited at Herbarium, Department of Botany, RTM Nagpur University Nagpur. Material: Tolbutamide as a standard (Aventis laboratory), biochemical estimation kits e.g. RBC diluting fluid, WBC diluting fluid, Drabkin’s reagent for haemoglobin estimation (AGAPPE Diagnostics), Serum creatinine estimation kit (Biolab Diagnostic Pvt. Ltd.), total bilurubin estimation kit (Biolab Diagnostic Pvt. Ltd.), SGOT and SGPT estimation kit (Biolab Diagnostic Pvt. Ltd.) were used for biochemical estimation.