This article is protected by copyright. All rights reserved Article Fascaplysin induces caspase mediated crosstalk between apoptosis and autophagy through the inhibition of PI3K/AKT/mTOR signaling cascade in human leukemia HL-60 cells † Suresh Kumar, a,1 Santosh Kumar Guru, a Anup Singh Pathania, a,1 Sudhakar Manda, b,1 Ajay Kumar, a Sandip B Bharate, b Ram A Vishwakarma, b Fayaz Malik, a,1* Shashi Bhushan a,1, * 1 Academy of Scientific and Innovative Research (AcSIR) New Delhi 110001, India a Cancer Pharmacology Division, Indian Institute of Integrative Medicine, CSIR, Jammu-180001, India b Medicinal Chemistry Division, Indian Institute of Integrative Medicine, CSIR, Jammu-180001, India * Corresponding author: Shashi Bhushan, Ph.D. Senior Scientist, Cancer Pharmacology Division, Indian Institute of Integrative Medicine, CSIR, Canal Road, Jammu-180001, India Telephone: 091-191-2569000-9, Extension No. 291 E-mail: sbhushan@iiim.ac.in Abbreviations: AO, Acridine orange; ATG, autophagy related gene; LC3, microtubule- associated protein light chain 3; MTT, 3-(4,5-Dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide; PARP-1-1, Poly(ADP-Ribose) Polymerase-1; PI, propidium iodide; Rh-123, Rhodamine-123; ZVAD (zVAD fmk), benzyloxycarbonyl-Val-Ala-Asp fluoro-methyl-ketone; mTOR, The mammalian target of rapamycin; mpk, mg/kg Key words: Autophagy, Apoptosis, PI3K/AKT pathway, Caspases, Fascaplysin † This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: [10.1002/jcb.25053] Received 11 August 2014; Revised 5 December 2014; Accepted 18 December 2014 Journal of Cellular Biochemistry This article is protected by copyright. All rights reserved DOI 10.1002/jcb.25053