JOURNAL OF OIL PALM RESEARCH 24 (DECEMBER 2012) 1566 PRODUCTION OF LIVE FOOD FROM PALM OIL MILL EFFLUENT (POME) FOR CULTURE OF MARBLE GOBY LOO POH-LEONG*; CHONG VING-CHING** and VIKINESWARY SABARATNAM* ABSTRACT For the irst time a phototrophic bacterium, Rhodovulum sulidophilum grown in palm oil mill efluent (POME-PB) was successfully used as food for rotifers (Brachionus rotundiformis) and Artemia nauplii which were then fed to the larvae of the marble goby, Oxyeleotris marmorata. The cultivation of POME- PB is cheap and easy where it can be easily produced in sealed, plastic ziplock bags exposed to light, giving a harvestable biomass of 2.58 ± 0.34 g litre -1 after 60 hr post-inoculation. Rotifers fed the biomass of settled bacteria (sPOME-PB) had comparable mean density (221 ± 9 rotifers ml -1 ) as rotifers fed with microalgae Nannochloropsis sp. (212 ± 27 rotifers ml -1 ). However, unsettled bacterial culture (uPOME-PB) fed to rotifers resulted in higher rotifer densities of up to 898 ± 489 rotifers ml -1 . By feeding the marble goby larvae with rotifers and Artemia nauplii cultured in uPOME-PB, a mean survival of 81.9 ± 3.0% was obtained for 30-day post-hatch ish larvae. This survival was much higher than ish larvae given rotifers and Artemia nauplii fed sPOME-PB (42.5 ± 9.0%; 71.4 ± 20.5%) and Nannochloropsis sp. (46.8 ± 2.9%). POME-PB thus has the potential to be an aquaculture feed for the future. * Institute of Biological Sciences, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia. E-mail: chong@um.edu.my ** Institute of Ocean and Earth Sciences, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia. harvested and used as feed for fish and other cultured organisms. Single-cell proteins (SCP) and oils (SCO) are the current viable sources that include unconventionally exploited microorganisms such as heterotrophic dinolagellates, microheterotrophs and phototrophic bacteria (PB). Unlike microalgae, these organisms do not strictly need light and are more useful in bioremediating organic wastes, which if untreated, pollute the environment. One such waste is palm oil mill efluent (POME) generated in large volumes by Malaysia’s largest agroindustry that contributes to 3.3% of its gross domestic product. It is estimated that for every tonne of crude palm oil produced, an equivalent 2.5 t of POME is generated (Department of Environment, 1999). Yet POME is nutrient and lipid rich (Phang, 1990). A dual solution to overcome a potentially debilitating environmental problem caused by the palm oil industry and the woes of the aquaculture industry is to ind that one ‘magic bullet’ that could mitigate the organic pollution while turning it into a nutriment for ish. That magic bullet could be a INTRODUCTION Worldwide aquaculture produces 55.1 million tonnes of ish to supplement the ever increasing demand for fish food and protein, while wild catches continue to fall (FAO, 2010). The commercial production of artiical feed for cultured organisms however uses 71% and 90% of the world’s ish meal and ish oil production, respectively (Tacon and Metian, 2008), and it is projected that aquaculture feed production will face serious shortages in the near future as ish meal production leveled off and fell since 1995 (James and Geoff, 2001). The search is now for alternative sources of proteins and omega 3-rich oils that could be mass cultured cheaply, Keywords: palm oil mill efluent, phototrophic bacteria, rotifers, marble goby culture. Date received: 26 April 2012; Sent for revision: 6 June 2012; Received in final form: 6 September 2012; Accepted: 26 September 2012. Journal of Oil Palm Research Vol. 24 December 2012 p. 1566-1572 N-Poh Leong.indd 1566 12/31/12 4:49 PM