Animal Reproduction Science 124 (2011) 12–18 Contents lists available at ScienceDirect Animal Reproduction Science journal homepage: www.elsevier.com/locate/anireprosci Fixed-time AI protocols replacing eCG with a single dose of FSH were less effective in stimulating follicular growth, ovulation, and fertility in suckled-anestrus Nelore beef cows J.N.S. Sales a , G.A. Crepaldi a , R.W. Girotto b , A.H. Souza a , P.S. Baruselli a,∗ a Departamento de Reproduc ¸ ão Animal, FMVZ-USP, Rua Prof. Orlando Marques de Paiva 87, CEP: 05508-270, São Paulo, SP, Brazil b RG Genética Avanc ¸ ada, Rua 1, Sala 03, n ◦ 481, CEP: 78635-000, Água Boa, MT, Brazil article info Article history: Received 8 June 2010 Received in revised form 19 January 2011 Accepted 7 February 2011 Available online 13 February 2011 Keywords: FSH Bos indicus Ovulation Follicular growth Estrous synchronization abstract The aim of the present study was to evaluate the effects of a single treatment with FSH on diameter of the largest follicle and on conception rates of suckled Bos indicus beef cows submitted to timed artificial insemination (TAI). Four hundred fifty-six suckled anestrous Nelore beef cows at 30–60 days postpartum were assigned to treatments. At the first day of the estrous synchronization protocol (Day 0), all cows received a progesterone-releasing intravaginal device plus 2 mg of estradiol benzoate. On Day 8, cows were assigned to blocks according to the diameter of the largest follicle and then allocated to one of three treat- ment groups (Control, FSH, or eCG) within each block. Simultaneously to progesterone device withdrawal on Day 8, cows in the eCG treatment group (n = 150) received 300 IU of eCG and cows in FSH treatment group (n = 153) received 10 mg of FSH, and Control cows (n = 153) did not receive any additional treatment. Additional treatments with 150 g of cloprostenol and 1 mg of estradiol cypionate (EC) were also administered concurrently to progesterone device removal in all cows on Day 8. Two days later (D10), TAI and ovarian ultrasonic examinations to evaluate follicle size were performed in all cows. On Day 12, a subset of cows (n = 389) were submitted a second ultrasonic exam to confirm ovulation. Final follicular growth (mm/day) was less (P = 0.006) in both Control (0.95 ± 0.11) and in FSH-treated cows (0.90 ± 0.10) than in eCG-treated cows (1.40 ± 0.13). Interestingly, there was a treatment-by-BCS interaction in ovulation results (P = 0.03), in which, eCG treatment increased percentage of cows having ovulations with a lesser BCS. Similarly, there was a treatment-by-BCS interaction for conception (P = 0.04), where the eCG treatment increased fertility in cows with a lesser BCS. In conclusion, FSH failed to stimulate final follicular growth, ovulation, and conception rate in sucked-anestrous beef cows submitted to TAI as effectively as eCG. However, physiological effects of eCG seem to be more evident in cows with a lesser BCS. © 2011 Elsevier B.V. All rights reserved. ∗ Corresponding author. Tel.: +55 11 3091 7674; fax: +55 11 3091 7920. E-mail addresses: barusell@usp.br, znlogan@yahoo.com.br (P.S. Baruselli). 1. Introduction The majority of cattle in tropical and subtropical areas are of zebu (Bos indicus) origin. This is prob- ably determined by several physiological advantages encountered in zebu breeds over Bos Taurus includ- ing greater resistance to environmental challenges (high temperature and humidity) and greater adaptation to 0378-4320/$ – see front matter © 2011 Elsevier B.V. All rights reserved. doi:10.1016/j.anireprosci.2011.02.007