Ukaejiofo et al. Myelo-protective Activity of Fractions of Vitex doniana Sweet Leaves Extract in Wistar Rats, Medical Science, 2015, 18(71), 1-10, www.discovery.org.in © 2015 Discovery Publication. All Rights Reserved Page1 Myelo-protective Activity of Fractions of Vitex doniana Sweet Leaves Extract in Wistar Rats Ukaejiofo EO 1 , Ufelle SA 2҉ , Ghasi S 3 , Achukwu PU 4 , Udeani TKC 5 , Neboh EE 6 1. Professor, Department of Medical Laboratory Sciences, College of Medicine, University of Nigeria Enugu Campus, Nigeria 2. Senior Lecturer, Department of Medical Laboratory Sciences, College of Medicine, University of Nigeria Enugu Campus, Nigeria 3. Senior Lecturer, Department of Pharmacology and Therapeutics, College of Medicine, University of Nigeria Enugu Campus, Nigeria 4. Senior Lecturer, Department of Medical Laboratory Sciences, College of Medicine, University of Nigeria Enugu Campus, Nigeria 5. Senior Lecturer, Department of Medical Laboratory Sciences, College of Medicine, University of Nigeria Enugu Campus, Nigeria 6. Lecturer 1, Department of Medical Biochemistry, College of Medicine, Enugu State University of Science and Technology, Nigeria ҉ Corresponding author: Department of Medical Laboratory Sciences, College of Medicine, University of Nigeria Enugu Campus, Nigeria E-mail: silasufelle@yahoo.com Publication History Received: 14 June 2015 Accepted: 17 July 2015 Published: 7 October 2015 Citation Ukaejiofo EO, Ufelle SA, Ghasi S, Achukwu PU, Udeani TKC, Neboh EE. Myelo-protective Activity of Fractions of Vitex doniana Sweet Leaves Extract in Wistar Rats. Medical Science, 2015, 18(71), 1-10 ABSTRACT Myelo-protective activity of fractions of leaves extracts of Vitex doniana was investigated. Wistar rats (n=60) were intra-peritoneally induced for myelo-suppression with 3mg/kg bodyweight (b.wt) cyclophosphamide for 7 days. Column chromatography revealed 4 aqueous fractions (AF1, AF2, AF3, and AF4) and 2 methanol fractions (MF1 and MF2). The rats were divided into 6 groups and each group was orally administered with 100mg/kg b.wt of each fraction (group 1 =AF1, group 2=AF2, group 3= AF3, group 4=AF4, group 5= MF1 and group 6=MF2) for 14 days. Blood samples were collected on day 15 for haematological analysis. Data were analyzed using two-way analysis of variance in Graph pad prism version 5.03. Results revealed significantly increased values in AF1:WBC[x 10 9 /L]: 5.8 ± 0.4; Neutrophil [%]: 58 ± 2; AF3:WBC[x 10 9 /L]: 6.8 ±1.5; Neutrophil [%]: 67±2.5 and MF1: WBC[x 10 9 /L]: 6.2±0.5; Neutrophil [%]:65±2, compared to AF2:WBC[x 10 9 /L]: 2.4 ±0.4; Neutrophil [%]: 35±3; AF4:WBC[x 10 9 /L]: 2.35±0.4; Neutrophil [%]: 40±3; MF2:WBC[x 10 9 /L]: 3.5±0.4; Neutrophil [%]: 38±3 and control: WBC[x 10 9 /L]: 5.8±0.4; Neutrophil [%]: 60±3, (p<0.001). AF1, AF3 and MF1 also revealed significantly increased haemoglobin, haematocrit and RBC compared to other fractions and control (p<0.05). BMC (x10 6 /femur) increased in AF1: 15.30±0.7, AF3: 14.50± 1.5 and MF1: 10.50±1.2 compared to AF2: 3.1±0.9; AF4: 9.2±1.1 and MF2: 10.5±1.5 indicating myelo-protection from fractions AF1, AF3 and MF1. Key words: Myelo-protective, Fractions, Vitex doniana, Wistar rats Abbreviations: BMC-Bone marrow cells, WBC-White blood cells, AF-Aqueous fraction, MF-Methanol fraction Medical Science RESEARCH The International Weekly journal ISSN 2321 – 7359 EISSN 2321 – 7367