Extensive Journal of Applied Sciences Available online at www.ejasj.com ©2014 EJAS Journal-2014-2-2, 29-32 Microbiological Studies on the Wastewater Treatment and Agricultural Fertilization Mohamed Younes Abdelfatah Hassan*, Omkolthom Hassan Khatab 1 , Eglal Abd Alla Ghoneimy 2 ,Abo-Elnasr A.A 3 ,Hamdy Abdel-Azeim Hassan 4 1. Hellwan University-Faculty of Science- Botany and Microbiology Department 2. Al-Azhar University-Faculty of Science- Botany and Microbiology Department 3. Botany and Microbiology Department, Faculty of Science, Helwan University 4. Environmental Biotechnology Dept-Genetic Engineering and Biotechnology research Institute- Sadat city University Corresponding author: Mohamed Younes Abdelfatah Hassan ABSTRACT: The present study was conducted for sewage treatment using some Microorganisms (useful, safe and available in eco friendly form). Like Lactobacillus. Saccharomyces and were isolated from respective sources. The microbial consortium was formulated using molasses as medium and incubated at 37°C for 3 days. The sewage treatment was carried out with the addition of 0.5 ml/l of microorganism's solution under aerobic condition. TSS, TDS, pH and the untreated sewage causes foul smell (bad odor) were reduced while clarification of waste water was increased by respectively after 3 - 24 hours of treatment. The results showed that the formulated many of Microorganisms efficient for sewage treatment and thereby it reduced the environmental impact.Biological processes to remove physical, chemical and biological contaminants. Its objective is to produce an environmentally safe fluid waste stream (or treated effluent and a solid waste (or treated sludge suitable for disposal or reuse (usually as farm fertilizer With suitable technology.This study evaluates the efficacy of yeasts (Saccharomyces cerevisiae) and Lactobacillus isolated in the treatment of wastewater biologically.All the experiments were done in triplicates and were performed in (waste water treatment plant) at Elminia for drinking water and sanitation company Abuqurqass branch. Keywords: Microbiological Studies on the Wastewater Treatment INTRODUCTION The food and water we consume are often contaminated with a range of chemicals and heavy metals, such as lead, cadmium, arsenic, chromium, and mercury that are associated with numerous diseases. Although heavy- metal exposure and contamination are not a recent phenomenon, the concentration of metals and the exposure to populations remain major issues despite efforts at remediation. The ability to prevent and manage this problem is still a subject of much debate, with many technologies ineffective and others too expensive for practical large- scale use, especially for developing nations where major pollution occurs. This has led researchers to seek alternative solutions for decontaminating environmental sites and humans themselves. A number of environmental microorganisms have long been known for their ability to bind metals, but less well appreciated are human gastrointestinal bacteria. Species such as Lactobacillus, present in the human mouth, gut, and vagina and in fermented foods, have the ability to bind and detoxify some of these substances. This review examines the current understanding of detoxication mechanisms of lactobacilli and how, in the future, humans and animals might benefit from these organisms in remediation environmental contamination of food. (Gregor Reid 2012)* Sewage treatment is one of the major problems faced by municipalities. Sewage is the wastewater comprising 99.9% water and 0.1% solid particles. The domestic sewage has high amount of organic and inorganic pollutants [1]. The untreated sewage causes foul smell (bad odor) [2]. The improper disposal of sewage causes pollution and destroys the aquatic organisms due to high organic content and biological oxygen demand (BOD) concentration [3]. So, the sewage has to be treated to reduce the environmental impact. The chemically treated water causes harmful effects due to toxic chemicals than the organisms which are originally present in the sewage [4]. The organisms present in wastewater degrade organic matter [5] and helps for further treatment. In conventional treatment method, bacteria remove the organic content of wastewater but the solid particle remains as sludge. The sludge can be used as fertilizer or incinerated, disposed into ocean or landfill. The conventional sewage treatment processes are expensive to operate and maintain [6] and causes pollution.