Prosiding Seminar Kimia Bersama UKM-ITB VIII 9-11 Jun 2009 269 SYNTHESIS OF AMYLOSE AND AMYLOPECTIN BRUSHES ON SILICON WAFER SURFACE PRADANTI MANDIRI VIDYAWARDHANI 1 , JEROEN VAN DER VLIST 2 , KATJA LOOS 2 , SADIJAH ACHMAD 1 , DEANA WAHYUNINGRUM 1 1 Chemistry Study Program, Faculty of Mathematics and Natural Sciences, Bandung Institute of Technology, Indonesia 2 Department of Polymer Chemistry. Zernike Institute for Advanced Materials. University of Groningen. Nijenborgh 4. 9747 AG Groningen. The Netherlands e-mail: pradanti105@students.itb.ac.id ABSTRACT Polysaccharides are polymer from nature that has attracted new interest as advanced materials due to its biocompatibility, structure-forming capacity, and environmentally friendly property. One of its application fields is to synthesis polymer brushes from amylose and amylopectin. Polymer brushes can be obtained after three steps of reaction: modifying the surface of silicon wafers with (3- aminopropyl)trimethoxysilane (APTMS), grafting maltoheptaose as a primer to silicon surface, and enzymatic polymerization of the primer. To understand polymers property on silicon wafer surface, three different glucose-1-phosphate (G1P) concentrations have been used. According to IR spectrum and XPS data, amylose and amylopectin brushes have been successfully synthesized but cannot be distinguished. The roughness of the layer on the surface was determined by using AFM but it still cannot be concluded whether the concentration of G1P has an effect towards it. The thickness of the layer on the surface has been calculated but can only be applied to amylose brushes. Kinetics measurements showed that amylopectin wafers always grow faster than amylose wafers for every G1P concentration used. Determination of surface density of the amine group concludes that the amount of APTMS has been attached to silicon wafer was 1.86 molecule/nm 2 meanwhile maltoheptaose gives value of 0.45 molecule/nm 2 . Keywords: amylose, amylopectin, polymer brushes, enzymatic polymerization.