191 한국균학회지 The Korean Journal of Mycology A New Record of Neosartorya aureola Isolated from Field Soil in Korea Mahesh Adhikari 1 , Sangwoo Kim 1 , Dil Raj Yadav 1 , Changmu Kim 2 , Hyang Burm Lee 3 and Youn Su Lee 1 * 1 Division of Biological Resource Sciences, Kangwon National University, Chuncheon 24341, Korea 2 Biological and Genetic Resources Assessment Division, National Institute of Biological Resources, Incheon 22755, Korea 3 Division of Food Technology, Biotechnology & Agrochemistry, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea ABSTRACT : A new species of Neosartorya was recovered during investigation of the fungal community in soil samples collected from different locations in Korea; Neosartorya aureola KNU14-7 was isolated for the first time from field soil in Korea and identified based on the internal transcribed spacer region of rDNA and morphological characteristics. The species has not been officially reported from Korea and we report it here with description and figures. KEYWORDS : Molecular identification, Morphology, Neosartorya aureola Neosartorya is a widespread genus of the Trichocomo- ceae family . It is teloemorph of Aspergillus section Fumi- gati include many species which is important because of its pathogenic or allergenic to human [1]. Most members of the genus Neosartorya Malloch & Cain [2] in the Euro- tiales are of worldwide distribution and are very abun- dant, occurring nearly everywhere in soil, air, dust, food etc. [3-7]. A few Neosartorya species are mycotoxigenic and causal agents of human diseases like aspergillosis, osteomyelitis, endocarditis and mycotic keratitis. [8-16]. Certain species of the genus are used in the production of bioactive metabolites [17]. All of the Neosartyora spe- cies produce heat resistant ascospores that are frequently encountered in different food products and cause the spoilage of processed food products [18, 19]. Thus, there has been considerable recent interest of mycologists in working with Neosartorya aureola. During studies on the diversity of fungal communities in field soil of Gangwon-do, Korea, a species of Neosar- torya was encountered. Based on its morphological and molecular characteristics, this species was identified as N. aureola and named N. aureola KNU14-7. To the best of our knowledge this fungus has not been officially reported in Korea. Collection of soil samples and fungal isolation Soil samples were collected from different locations in Samcheok city, Korea in 2014. Soil samples were taken from (0~15 cm) depth, air dried and stored in plastic bags at 4 o C until used. The fungi were isolated by con- ventional dilution method and supplemented with 100 μg chloramphenicol per mL potato dextrose agar (PDA; Difco, Detroit, USA) and grown for 7 days at 28 o C until the growth of colonies was observed. ITS, β-tubulin and calmodulin gene sequencing analysis Genomic DNA of the strain was extracted using DNeasy Plant Mini Kit (QIAGEN, Germantown, MD, USA) follow- ing the manufacturer’s instructions. The internal transcri- bed spacer (ITS) regions, including the 5.8S were ampli- fied with the primers ITS1 and ITS4 [20]. For the sequen- cing of partial β-tubulin and calmodulin genes, the seg- ments of β-tubulin and calmodulin were amplified with the primers BT2a and BT2b and cmd5 and cmd6 [21, Research Note *Corresponding author E-mail: younslee@kangwon.ac.kr Received June 5, 2015 Revised June 13, 2015 Accepted September 8, 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http:// creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Kor. J. Mycol. 2015 September, 43(3): 191-195 http://dx.doi.org/10.4489/KJM.2015.43.3.191 pISSN 0253-651X • eISSN 2383-5249 © The Korean Society of Mycology