10 A TWO-DIMENSIONAL LIQUID MASS MAPPING TECHNIQUE FOR BIOMARKER DISCOVERY David M. Lubman Department of Chemistry, Department of Surgery, Comprehensive Cancer Center, The University of Michigan, Ann Arbor, MI 48109, USA Nathan S. Buchanan, Paweena Kreunin, and Yanfei Wang Department of Chemistry, The University of Michigan, Ann Arbor, MI 48109, USA Fred R. Miller Karmanos Cancer Institute, Wayne State University, Detroit, MI 48201, USA Kathleen Cho and Rong Wu Department of Pathology, The University of Michigan, Ann Arbor, MI 48109, USA Steven Goodison Department of Surgery, University of Florida, Jacksonville, FL 32209, USA Timothy J. Barder Eprogen, Inc., Darien, IL 60561, USA 10.1 INTRODUCTION Q1 Identification and validation of biomarkers predictive of disease, particularly cancer, is a significant and expanding area in clinical research. Quality cancer biomarkers should facilitate early detection and diagnosis of the disease, with more specific markers used for classification (such as grade/stage) or subtype determination of the Multidimensional Liquid Chromatography: Theory and Applications in Industrial Chemistry and the Life Sciences, Edited by Steven A. Cohen and Mark R. Schure. Copyright Ó 2008 John Wiley & Sons, Inc. 221