543 Review www.expert-reviews.com ISSN 1746-9899 © 2008 Expert Reviews Ltd 10.1586/17469899.3.5.543 To understand the histiogenesis of lympho- mas and to define distinct lymphoma entities, the current WHO classification emphasizes an approach whereby the clinical characteris- tics are correlated with distinct morphological, immunophenotypical, and genotypical features of each neoplasm [1] . For each lymphoma entity, a putative cell of origin is postulated. Intraocular lymphomas are heterogeneous and have been subsumed for many years under the vague title ‘primary intraocular lymphoma’ (PIOL). In fact, intraocular lymphomas repre- sent several distinct entities, arising in different structures of the eye and have differing clinical courses; therefore, more specific terminology is warranted (BOX 1 & TABLE 1) . In this review, the pathology and molecular biology of the main types of intraocular lymphoma are summarized. Where possible, the cell from which the neoplas- tic population has arisen will be proposed for the main intraocular lymphoma entities. Retinal lymphoma Most intraocular lymphomas occur in the retina, usually with vitreous involvement [2] . Occasionally, retinal lymphoma occurs without visible vitreous opacities and, less commonly, lymphomatous vitreous infiltrates are seen in the absence of any obvious retinal disease. For ease of reference, the three different clinical variants of retinal and vitreal lymphoma will be described here under the term ‘retinal’ lymphoma (BOX 1). Clinical features Retinal lymphoma is a high-grade malignancy that is often associated with cerebral disease. It may be primary or secondary to CNS disease or may present simultaneously [3–5] . Retinal lymphoma most often affects elderly patients; however, it is also seen in younger individuals, particularly those who are immunocompromised following HIV infection or organ transplantation. Inexplicably, the incidence of retinal lymphoma is increasing worldwide, even in immunocom- petent patients [6] . Retinal lymphoma is usually bilateral (60–90% of patients) but is often very asymmetrical and apparently unilateral at initial presentation [2] . Approximately 80% of patients with reti- nal lymphoma subsequently develop CNS Sarah E Coupland and Bertil E Damato Author for correspondence Department of Cellular and Molecular Pathology, University of Liverpool, Duncan Building, Daulby Street, Liverpool, L69 3GA, UK Tel.: +44 151 706 5885 Fax: +44 151 706 5859 s.e.coupland@liverpool.ac.uk This article describes recent advances in the understanding of the molecular genetics of intraocular lymphomas. These comprise vitreoretinal lymphoma, primary choroidal lymphoma, primary iris lymphoma and secondary choroidal lymphomas. Following WHO principles, their classification is now based on morphological, immunophenotypical and genotypical features. Retinal lymphoma is a high-grade lymphoma, usually of B-cell type, and is associated with a poor prognosis because of CNS involvement. Immunophenotyping and somatic mutation analysis suggest derivation from early postgerminal center B cells. Chromosomal translocation data suggest that some arise from germinal center B cells, which may have a better prognosis. Primary choroidal lymphoma is a low-grade B-cell lymphoma similar to nonocular, extranodal marginal zone B-cell lymphomas (EMZL). The putative cell of origin is a postgerminal center (memory) B cell. Primary iridal lymphomas are rare, with an equal distribution of B- and T-cell types and with a variable clinical course, most patients succumbing to systemic dissemination. Only one case of primary ciliary body lymphoma has been reported. Secondary choroidal lymphomas/leukemias tend to represent advanced systemic disease. Progress has been made in understanding the histiogenesis of intraocular lymphoma. Further investigations, such as gene expression profiling, are required to identify oncogenic pathways, to enhance prognostication and improve therapy. KEYWORDS:฀choroidal฀lymphoma฀•฀ciliary฀body฀lymphoma฀•฀DLBCL฀•฀EMZL฀•฀intraocular฀lymphoma฀•฀iris฀lymphoma฀ •฀retinal฀lymphoma฀•฀vitreal฀lymphoma฀•฀vitreoretinal฀lymphoma฀•฀uveal฀lymphoma฀ Molecular pathology of intraocular lymphomas Expert Rev. Ophthamol. 3(5), 543–551 (2008) For reprint orders, please contact reprints@expert-reviews.com