Journal of Sustainable Development of Energy, Water and Environment Systems Year XXXX Volume X, Issue Y, pp xx-yy Page assigned by journal Using R-Software to Statistically Determine the Effects of Temperature, Pressure and Number of Cycles on Partially Diluted Yeast from High-Pressure Homogenizer (HPH) Leonard E. N. Ekpeni 1 , Roberto F. Martinez 2 , Marina C. Bobadilla 3 , Ruben L. Lorza 3 , Joseph Stokes 1 , Abdul-G. Olabi 4 1 Biofuel Research Group, School of Mechanical & Manufacturing Engineering, Dublin City University, Dublin 9, Ireland. e-mail: leonard.ekpeni3@mail.dcu.ie 2 Department of Material Science, University of Basque Country UPV/EHU, Bilbao Spain 3 Department of Mechanical Engineering, University of La Rioja, Spain 4 Institute of Energy & Engineering Technology, University of the West of Scotland, Paisley, PA1 2BE, Scotland, UK ABSTRACT Biomass as a renewable energy source (RES) which have its end uses as bio-fuel or bio-energy for electricity generation, heating in the form of combined heat and power (CHP) as well as fuel for transportation is therefore considered as a valuable source of energy as an alternative to the use of fossil fuel. Biogas production through high pressure homogenizer (HPH) has now taken a new turn since the advent and improvement of biogas for energy uses as one of the renewable energy sources [RES], but this improvement has been impeded through some of the operating parameters. On consideration in this study will be the conventional GYB40-10S 2-Stage Homogenizing Valves with 100 MPa (max.). Against this background, work on the study will be to statistically determine the effects of temperature, pressure and number of cycles on partially diluted yeast from high pressure homogenizer (HPH) using the R-software for its analysis. KEYWORDS HPH; RES; CHP; Yeast; Biomass; R-Software; Biogas Production INTRODUCTION Energy need of the current 21 st century, has called for a more drastic action being taken so as to save the climate and environment from further attack catastrophically, as this has been considered so detrimental to the people living on the earth surface. As energy use can never be undermined due to its importance in our daily activities, alternative measures are all being considered to take the current situation of fossil fuels and nuclear energy usage. In 2007, it was fully acknowledged that the change in the global climate and its relation to the burning of fossil fuel is one of the main challenges of the world today [1]. The overall idea here is therefore a need for the enhancement of global strategies for energy security and mitigation of CO 2 -energy related emissions, for which the salient strategies include, inter alia, the need for: increased energy efficiency, increased use of clean fossil energy and increased use of renewable energy [2]. Strategically, as renewable energy sources (RES) has therefore comes as a way in improving this energy need for the world growing population, biomass choice in this