The giant Antarctic toothfish Dissostichus mawsoni
is a large perciform fish belonging to the suborder
Notothenioidei endemic to the inshore waters of the
Southern Ocean encircling Antarctica (Eastman, 1993).
It is very large fish often reaching 1 m in length and
weighing between 34 and 55 kg (Dewitt et al., 1990),
and living up to 50 years (Horn et al., 2003). The mean
water temperature in the Southern Ocean year-round
is -1.86°C, which is at or near the freezing point of
seawater (Hunt et al., 2003). The toothfish is a well-
studied animal displaying a number of biochemical
and physiological adaptations to the cold (Williams
et al., 1985; Eastman, 1993; Chen et al., 1997; Metcalf
et al., 1999; Pointer et al., 2005). As a large fish, it has a
large prominent eye (Fig. 5.1). Interestingly, no reports
of lens cataracts have been noted in the literature
during the approximately 40 years of study. Because
of unique habitat of the toothfish, it is worth placing
this animal in context of existing model systems, both
from the perspective of its environment and its rele-
vant general biology if we are to properly exploit the
toothfish as a new model system for lens biology.
THE ANTARCTIC ENVIRONMENT
The Antarctic continent covers approximately 14 mil-
lion km
2
and is the Southern-most of the earth’s land
masses. It is oceanographically isolated from all other
continental land masses by large amounts of open
water. This isolation has allowed for the development of
the Antarctic circumpolar current (ACC). The ACC has
the largest volume of any oceanic current and extends
from the ocean surface to the sea-floor (2000 ~ 4000 m).
The ACC varies from 200 km to 1000 km wide (slightly
deflected and compressed by the tip of South America)
thereby representing a true physical barrier separating
the fishes of the Southern Ocean from those of the rest
of the world (Eastman, 1993).
The temperature on the Antarctic continent itself
is at or below 0°C, with the world’s lowest recorded
temperature of -89.6°C reported at Vostok Station
(USSR). In addition to being extremely cold year-round
there is very little yearly precipitation, 40~100 cm per
year on the coast with trace amounts at the South Pole
The Antarctic Toothfish: A New
Model System for Eye Lens Biology
Andor J.Kiss
Laboratory for Ecophysiological Cryobiology, Department of Zoology,
Miami University, Oxford, OH 45056, USA
The Antarctic Environment 48
Toothfish Biology 49
Lens Biochemistry 50
Lens Crystallin cDNA Sequences 52
Other Aspects of Toothfish Eye Biology 53
Strengths of the Toothfish as a Model System 53
References 54
OUTLINE
5
CHAPTER
Animal Models in Eye Research © 2008, Elsevier Ltd. 48