!"#$%$&!$ ’() (* ++ ,-"%,#. *!,. / / , / 0 * 1 2 34 * 5 67 & , 82/ 09/ :2/ +( / 65 4 62 ;/ 4 * 5 /2/ 09/ :2/ +( / 65 4 62 ;/ 4 & :2/ 6 09/ :2/ +( / 65 4 62 ;/ 4 Abstract: Objective of this study are toassessthe effect of polyphenol from Rambutan Peel Extract (RPE) on protein profile and serum lipid in normal and obesity rat model. Normal and obesity rat model have been treated with polyphenol from rambutan peel extract for 12 weeks. Rat were divided into 2 major groups based on their weight which normal rat and obesity model rat respectively have average weight 180&200g and 360&380g. This treatment were divided into minor groups which were placebo (without treatment), treatment with ellagic acid and polyphenol (dosage 15 mg/kg BW; 30 mg/kg BW and 60 mg/kg BW). Polyphenol were delivered by oral administration for 12 weeks. Protein profile on visceral fats wereidentified using SDS page and serum lipid wasevaluated based onbody weight, weight on visceral fat andtriglycerides. Polyphenol were significantly inhibit body mass gain on treatment polyphenol 30mg/kg BW. Mass of visceral fats were not different on treatment and non&treatment rat, however significantly different on normal and obesity rat model. Level of triglycerides were less on rat treated with polyphenol 30mg/kg BW.Protein profile characteristic normal rat and obesity&model rat were in range between117&20 kDa. The amount of band protein were found in normal rat were less than the amount of protein on obesityrat model. There was difference molecular weight at protein density 57kDa for obesity rat model which has been treated with polyphenol. Polyphenol from RPEdecreased triglyceride level and amount of protein band on induced high calorie diet rat. Key word: protein profile, polyphenol, obesity rat model. Introduction Protein is a macromolecule which constructs and dominates more than half of cell structure. Proteins has some functions in cell, such as determine cell’s size and structure, facilitates cell to cell communication and biochemical reaction in cell. Protein has close relation with physiological process in the body. The variance and number of protein is a key factor which have responsibility for biological process in organism. Physiologic functions of Protein are as a catalyst, carrier, messenger, etc. Protein consistedof the series combination from amino acid. Each protein has certain number and sequence of particular amino acid 1 . Protein expression is a series of complex process which involve many factors. Genetic expression process is started and being arranged since pre&transcription initiation to translation 1 . The result of gene