Research Article Open Access Med Aromat Plants ISSN: 2167-0412 MAP, an open access journal Open Access Research Article Medicinal & Aromatic Plants ISSN: 2167-0412 M e d i c i n a l & A r o m a t i c P l a n t s Deressa et al., Med Aromat Plants 2015, 4:4 http://dx.doi.org/10.4172/2167-0412.1000208 Volume 4 • Issue 4 • 1000208 *Corresponding author: Deressa T, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, P.O.Box. 196, Gondar, Ethiopia. Tel: 251911738270; E-mail: tekalign09@gmail.com Received August 10, 2015; Accepted September 07, 2015; Published September 10, 2015 Citation: Deressa T, Tamiru T, Biadgo B, Belete D, Zewdu S, et al. (2015) Antimicrobial Potentials of Apis Multilora Honey in Combination with Coffee and Cinnamon Extracts against Common Human Pathogenic Bacteria. Med Aromat Plants 4: 208. doi:10.4172/2167-0412.1000208 Copyright: © 2015 Deressa T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Antimicrobial Potentials of Apis Multiflora Honey in Combination with Coffee and Cinnamon Extracts against Common Human Pathogenic Bacteria Deressa T 1 *, Tamiru T 2 , Biadgo B 1 , Belete D 1 , Zewdu S 1 , Mengistie S 1 , Endris M 1 and Tessema B 1 1 School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, P.O.Box. 196, Gondar, Ethiopia 2 Department of Biotechnology, Faculty of Natural and Computational Science, University of Gondar, P.O.Box. 196, Gondar, Ethiopia Keywords: Honey, Cinnamon, Cofee, Antimicrobial activity Introduction Infectious diseases represent an important cause of morbidity and mortality worldwide [1,2]. Chemotherapy of infected individuals with antimicrobial drugs is one of the widely used strategies for the control of infectious diseases [3-5]. However, the recent upsurge of antimicrobial drug resistance and its spread has posed a unique challenge to the global infectious diseases control program [3-5]. Further, afordability and accessibility of newer pharmaceutical antimicrobials are issues of major concern in developing countries, especially in rural areas. herefore, a search for safe, cheaper and efective antimicrobial agents is of urgent necessity to cope with this global challenge. With this regard, traditional medicinal plants are one of the potential sources of alternative antimicrobial agents that can be used for treatment of various ailments [6,7]. Medicinal plants have played crucial roles in traditional health care system since the origin of mankind. here has been a worldwide increase in public interest toward traditional remedies, involving natural products, in the past decades [8]. Presently, it is estimated that up to 80% of population in developing countries rely on herbal medicines to meet their primary health care needs [9] and herbal remedies are being available in drug stores and supermarkets [8]. In Ethiopia, traditional medicines continue to be an important segment of primary health care to the majorities of rural population [10]. his wide usage of traditional remedies among the population of Ethiopia could be in part attributed to their probable eicacies against some diseases, cultural acceptability, and their accessibility and afordability compared to allopathic medicines. Despite the wide usage, information regarding the safety and in vivo or in vitro eicacies of Ethiopian traditional medicines are very limited. hus, careful screening of the safety and eicacies of these traditional medicines is of paramount importance. Honey has been used as a medicine since ancient times and is still being used in many traditions. A number of studies revealed the antimicrobial potential of honey for treatment of ulcers, bed sores, wound infecting methicillin-resistant Staphylococcus aureus and bacterial gastroenteritis in infants [11,12]. In Ethiopia, honey has been and being used traditionally for treatments of respiratory infections, coughs and gastrointestinal complaints either singly or in combination with other beverages like cofee. Similarly, cofee is used for treatment of respiratory infections and stomach pain in combination with honey and/or Cinnamon barks. Extracts of cofee have been reported to Abstract Background: Traditional medicines have been used widely by the people of Ethiopia for treatment of various ailments. However, scientiic evidences regarding their antibacterial potential are scarce. The present study aim to assess an in vitro antibacterial activity of Apis multilora honey in combination with coffee and cinnamon bark extracts against the standard and clinical isolates of human pathogenic bacteria. Methods: Antimicrobial activities of honey and the extracts were tested against Escherichia coli, Citrobacter species, Staphylococcus epdermidis, Staphylococcus aureus, Pseudomonas aeruginosa (ATCC 27853) and Staphylococcus aureus (ATCC 2923). Agar well diffusion and micro-well broth dilution techniques were used to determine the antibacterial activity and minimum inhibitory concentration. Result: Honey exerted a maximum bacterial inhibition against Citrobacter species and E. coli (29 mm). Coffee extracts displayed best antibacterial activity against S. aureus (25-26 mm) and cinnamon extract exhibited the maximum inhibitory effect against S. epidermidis (31 mm). The combination of honey with cinnamon was most effective against P. aeruginosa (27 mm), whereas the combination of honey with coffee and cinnamon extracts was most effective against S. aureus ATCC 2923 (35 mm). The antibacterial activity exerted by a reference antibiotic ceftraxone against different test strains ranged from 24-37 mm. Conclusion: Coffee and cinnamon extracts, and honey have demonstrated a broad spectrum antibacterial effect. Our data indicating that these natural products have potential to be used as alternative antimicrobials for treatment of pathogenic bacteria. Thus, we recommend further investigations of each extract to elucidate bioactive compounds responsible for the observed antibacterial activity.