Int.J.Curr.Microbiol.App.Sci (2014) 3(8) 73-83 73 Original Research Article Experimental study on the effect of microbiological interactions in the scorpion Tityus serrulatus Jose Brites-Neto 1,2* , Héber Escudero Bombonato 1 , Keila Maria Roncato Duarte 3 , Francisco de Assis Baroni 4 , Celso Guimarães Barbosa 5 , Simone Possedente de Lira 2 and Luiz Humberto Gomes 2 1 Health Department of Americana/SP; 2 Department of Exact Sciences/ESALQ/USP, Brazil 3 Institute of Animal Science of Nova Odessa/SP, Brazil 4 Department of Veterinary Microbiology and Immunology/UFRRJ, Brazil 5 Department of Mathematics/Institute of Exact Sciences/UFRRJ, Brazil *Corresponding author ABSTRACT Introduction The Phylum Arthropoda represents more than 80% of the total existing species including the arachnidan (Subphylum Chelicerata - Class Arachnida) where the scorpions are classified (Order Scorpiones). The origin of scorpions is dated from 400 million years ago. The species survived to all the major cataclysms, it was on earth before the dinosaurs. Scientific evidences appoint that scorpions were from aquatic life, as the specie Praearcturus Gigas, a great predator with around one meter long. ISSN: 2319-7706 Volume 3 Number 8 (2014) pp. 73-83 http://www.ijcmas.com Keywords Biological control, entomo- pathogenic fungi, fungal repellency, antibiosis, disharmonious interaction. The urban sprawl combined with extinction of rural areas and nature reserves in cities favored the development of many pests adapted to sinanthropic ecosystem where the yellow scorpion (Scorpiones: Buthidae, Tityus serrulatus, Lutz and Mello, 1922) stood out as urban pests causing serious human accidents of public health importance. The use of fungal pathogens for biological control of pests and the richness of its biodiversity in species and strains of importance in the research for pathogens in invertebrates have developed a great potential for biological control of venomous arthropods. In the period 2006 to 2013, were implemented a mechanical method of catching scorpions in infestation foci of cemetery in Americana, São Paulo, Brazil, using a procedure that consisted in opening tombs to collect scorpions housed internally. During this activity has been found that in many tombs had no scorpions there was a contamination with environmental characteristics of filamentous fungi, compared to other large amounts of them in the absence of fungal growth. Based on these field observations, we performed a bioassay for analysis of possible interactions of microorganisms isolated from these sites of infestation, which would determine repellency or death of specimens of scorpions kept in an experimental system monitored.