ORIGINAL PAPER Do multi-use cellulosic textiles provide safe protection against the contamination of sterilized items? B. Rogina-Car • A. Budimir • V. Turcic • D. Katovic Received: 8 October 2013 / Accepted: 7 February 2014 Ó Springer Science+Business Media Dordrecht 2014 Abstract The aim of this study was to determine whether multiple use cellulosic medical textiles (cot- ton blends, Tencel Ò ) could provide protection against contamination after sterilization, regardless of the barrier system of only qualified materials, as per EN 868-2, used in the process. New methods for testing permeability and durability of the microbial barrier cellulosic textiles were developed. The most resistant endospores of two apathogenic bacteria of the Bacill- lus genus (Geobacillus stearothermophilus and Bacil- lus atrophaeus) were used. Testing was conducted after 1, 10, 20, 30 and 50 washing and sterilization cycles under real hospital conditions of the University Hospital Centre Zagreb. The retention period of the microbial barrier of the diagonally packaged packages (one layer; EN ISO 11607-1:2009) after sterilization was tested after the time period of 1, 2 and 3 months of storage under controlled conditions. Bacterial perme- ability occurred in cellulosic medical textiles when they were contaminated with an extremely high quantity of aerobe bacterial spores. During the testing of microbial barrier durability, the package remained uncontaminated after 1, 2 and 3 months of storage. Medical cellulose textiles used under real hospital conditions functioned properly as a microbial barrier system after 50 cycles of washing and sterilization and 3 months of storage, as the sterilized content was not contaminated at all; they could be used as a microbe barrier system for packing in sterilization, regardless of the fact that they did not meet the standard EN 868-02:2009 Packaging materials for terminally ster- ilized medical devices. Part 2: sterilization wrap— requirements and test methods or the International standard, for example EN ISO 11607-1:2009 Packag- ing for terminally sterilized medical devices, part 1: requirements for materials, sterile barrier systems and packaging systems. Keywords Cellulose Á Multiple use medical textiles Á Microbial barrier Á Sterilization Á Geobacillus stearothermophilus Á Bacillus atrophaeus B. Rogina-Car (&) Department of Clothing Technology, Faculty of Textile Technology, University of Zagreb, Prilaz baruna Filipovica 28a, 10000 Zagreb, Croatia e-mail: beti.rogina-car@ttf.hr A. Budimir Department of Clinical and Molecular Microbiology, University of Zagreb, Zagreb, Croatia V. Turcic Clinical Department for Sterilization and Medical Surveillance of Employees, University Hospital Centre Zagreb, Zagreb, Croatia D. Katovic Department of Textile Chemistry and Ecology, Faculty of Textile Technology, University of Zagreb, Prilaz baruna Filipovica 28a, 10000 Zagreb, Croatia 123 Cellulose DOI 10.1007/s10570-014-0199-1