Supplemental Data S1 Virulence Is Positively Selected by Transmission Success between Mammalian Hosts Mark E. Wickham, Nat F. Brown, Erin C. Boyle, Brian K. Coombes, and B. Brett Finlay Supplemental References S1. Deng, W., Puente, J.L., Gruenheid, S., Li, Y., Vallance, B.A., Vazquez, A., Barba, J., Ibarra, J.A., O’Donnell, P., Metalnikov, P., et al. (2004). Dissecting virulence: Systematic and func- tional analyses of a pathogenicity island. Proc. Natl. Acad. Sci. USA 101, 3597–3602. S2. Gruenheid, S., Sekirov, I., Thomas, N.A., Deng, W., O’Donnell, P., Goode, D., Li, Y., Frey, E.A., Brown, N.F., Metalnikov, P., et al. (2004). Identification and characterization of NleA, a non-LEE-encoded type III translocated virulence factor of en- terohaemorrhagic Escherichia coli O157:H7. Mol. Microbiol. 51, 1233–1249. S3. Mundy, R., Petrovska, L., Smollett, K., Simpson, N., Wilson, R.K., Yu, J., Tu, X., Rosenshine, I., Clare, S., Dougan, G., et al. (2004). Identification of a novel Citrobacter rodentium type III secreted protein, EspI, and roles of this and other se- creted proteins in infection. Infect. Immun. 72, 2288–2302. S4. Kelly, M., Hart, E., Mundy, R., Marches, O., Wiles, S., Badea, L., Luck, S., Tauschek, M., Frankel, G., Robins-Browne, R.M., et al. (2006). Essential role of the type III secretion system ef- fector NleB in colonization of mice by Citrobacter rodentium. Infect. Immun. 74, 2328–2337. S5. Wickham, M.E., Lupp, C., Mascarenhas, M., Vazquez, A., Coombes, B.K., Brown, N.F., Coburn, B.A., Deng, W., Puente, J.L., Karmali, M.A., et al. (2006). Bacterial genetic determinants of non-O157 stec outbreaks and hemolytic-uremic syndrome after infection. J. Infect. Dis. 194, 819–827. S6. Guttman, J.A., Li, Y., Wickham, M.E., Deng, W., Vogl, A.W., and Finlay, B.B. (2006). Attaching and effacing pathogen-induced tight junction disruption in vivo. Cell. Microbiol. 8, 634–645. S7. Guttman, J.A., Samji, F.N., Li, Y., Deng, W., Lin, A., and Finlay, B.B. (2007). Aquaporins contribute to diarrhoea caused by at- taching and effacing bacterial pathogens. Cell. Microbiol. 9, 131–141. S8. Nagai, T., Abe, A., and Sasakawa, C. (2005). Targeting of en- teropathogenic Escherichia coli EspF to host mitochondria is essential for bacterial pathogenesis: Critical role of the 16th leucine residue in EspF. J. Biol. Chem. 280, 2998–3011. S9. Hardwidge, P.R., Deng, W., Vallance, B.A., Rodriguez- Escudero, I., Cid, V.J., Molina, M., and Finlay, B.B. (2005). Mod- ulation of host cytoskeleton function by the enteropathogenic Escherichia coli and Citrobacter rodentium effector protein EspG. Infect. Immun. 73, 2586–2594. S10. Tu, X., Nisan, I., Yona, C., Hanski, E., and Rosenshine, I. (2003). EspH, a new cytoskeleton-modulating effector of enterohae- morrhagic and enteropathogenic Escherichia coli. Mol. Micro- biol. 47, 595–606. S11. Ma, C., Wickham, M.E., Guttman, J.A., Deng, W., Walker, J., Madsen, K.L., Jacobson, K., Vogl, W.A., Finlay, B.B., and Vallance, B.A. (2006). Citrobacter rodentium infection causes both mitochondrial dysfunction and intestinal epithelial barrier disruption in vivo: Role of mitochondrial associated protein (Map). Cell. Microbiol. 8, 1669–1686. S12. Kanack, K.J., Crawford, J.A., Tatsuno, I., Karmali, M.A., and Kaper, J.B. (2005). SepZ/EspZ is secreted and translocated into HeLa cells by the enteropathogenic Escherichia coli type III secretion system. Infect. Immun. 73, 4327–4337. S13. Deng, W., Vallance, B.A., Li, Y., Puente, J.L., and Finlay, B.B. (2003). Citrobacter rodentium translocated intimin receptor (Tir) is an essential virulence factor needed for actin condensa- tion, intestinal colonization and colonic hyperplasia in mice. Mol. Microbiol. 48, 95–115.