Fitoterapia 75 (2004) 221–224 0367-326X/04/$ - see front matter  2004 Elsevier B.V. All rights reserved. doi:10.1016/j.fitote.2003.12.010 Short report Antioxidant activity of Citrus paradisi seeds glyceric extract Laura Giamperi, Daniele Fraternale, Anahi Bucchini, Donata Ricci* Istituto di Botanica e Orto Botanico’Pierina Scaramella’, Facolta di Farmacia, ` Universita degli Studi di Urbino, Via Bramante 28, 61029, Urbino, Italy ` Received 15 September 2003; accepted 16 December 2003 Abstract The antioxidant activity of Citrus paradisi (grapefruit) seeds glyceric extract dissolved in ethanol and in aqueous media was evaluated using three different methods: evaluation by DPPH assay, by 5-lipoxygenase assay and by luminolyxanthineyxanthine oxidase chemilu- minescence assay. The total phenolic content was determined by the Prussian Blue method opportunely modified. The grapefruit seeds glyceric extract utilized as aqueous solutions demonstrated antioxidant properties better than those displayed by alcoholic solutions.  2004 Elsevier B.V. All rights reserved. Keywords: Citrus paradisi; Antioxidant activity; Flavonoids Plant. Citrus paradisi Macf. (Rutaceae), evergreen tree originally of Asiatic South West (Tropical Asia, West India). Uses in traditional medicine. The fruit, a yellow hesperidium can be used to prepare different cosmetic products to tone up the epidermis, to treat acne and the flushed epidermis w1x. The oil from peel showed an inhibitory activity against *Corresponding author. E-mail address: d.ricci@uniurb.it (D. Ricci).