Supplementary Material Current Medicinal Chemistry, 2013, Vol. 20, No. 26 i Supporting Information Biophysical Characterization of Glycodendrimers As Nano-carriers for HIV Peptides M. Ionov* ,1 , K. Ciepluch 1 , B.R. Moreno 2 , D. Appelhans 2 , J. Sánchez-Nieves 3,4 , R. Gómez 3,4 , F.J. de la Mata 3,4 , M.A. Muñoz-Fernández 4,5 and M. Bryszewska 1 1 Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Poland; 2 Leibniz Institute of Polymer Research, Hohe Straße 6, D-01069 Dresden, Germany; 3 Dpto. de Química Orgánica y Química Inorgánica, Universidad de Alcalá, Campus Universitario, E-28871 Alcalá de Henares (Madrid), Spain; 4 Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN); 5 Laboratorio Inmu- noBiología Molecular, Hospital General Universitario Gregorio Marañón, Madrid, Spain MATERIALS AND METHODS All reactions were carried out under inert atmosphere and solvents were purified from appropriate drying agents. NMR spectra were recorded on a Varian Unity VXR-300 (300.13 ( 1 H), 75.47 ( 13 C), MHz) or on a Bruker AV400 (400.13 (1H), 100.60 ( 13 C), 79.49 ( 29 Si) MHz). Chemical shifts () are given in ppm. 1 H and 13 C resonances were measured relative to solvent peaks considering TMS = 0 ppm, meanwhile 29 Si resonances were measured relative to external TMS. When necessary, as- signment of resonances was done from gHSQC, gHMBC, gCOSY and gNOESY NMR experiments. Glycodendrimer charac- terization by NMR study: The NMR measurements were performed using a Bruker Avance III 500 NMR spectrometer operat- ing at 500.13 MHz for 1 H. The solvent was used as lock and internal standard (DMSO-d 6 :  ( 1 H) = 2.50 ppm). Spectra recorded from D 2 O solutions were referenced on external sodium 3-(trimethylsilyl)-3,3,2,2 tetradeuteropropionate in D 2 O ( ( 1 H) = 0 ppm. Elemental analyses were performed on a Perkin-Elmer 240C. Mass Spectra were obtained from an Agilent 6210 (ESI). Compounds 5-hexynoic acid (C 5 H 7 CO 2 H) and sodium ascorbate (Aldrich), and CuSO 4 (Panreac) were obtained from commer- cial sources. Compounds N 3 G 2 (NHBoc) 4 were synthesized as reported. The synthesis of the hybrid glycodendrimers HGD-3 and HGD-6 is summarized in Figure 1-SI. Key step for synthesizing HGD-3 and HGD-6 is the successful decoration of DS-PPIg4-3(NH 2 ) and DS-PPIg4-6(NH 2 ) with BOP-activated 2 nd genera- tion carbosilane HO 2 C-G 2 -(NHBoc) 4 dendrons. DS-PPIg4-3(NH 2 ) and DS-PPIg4-6(NH 2 ) were realized and characterized as described before (Vacas Córdoba et al., Nanomed. Nanotechnol. Biol. Med. (2013), http://dx.doi.org/10.1016/j.nano.2013.03.004). Furthermore, the number of coupled C 18 -NH 2 on PPIg4 scaffold was determined from the 1 H NMR spectrum of the final DS-PPIg4-X(NH 2 ) (with X = 3 and 6) in D 2 O. Degree of substitution of coupled C 18 -NH 2 on PPIg4 scaffold was in the desired range as indicated in Figure 1-SI. *Address correspondence to this author at the Department of General Biophysics, University of Lodz, Pomorska st. 141/143, 90-236, Lodz, Poland; Tel: +48426354144; Fax: +48426354474; E-mail: maksion@biol.uni.lodz.pl