ARVO 2013 Annual Meeting Abstracts by Scientific Section/Group - Cornea ©2013, Copyright by the Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Go to iovs.org to access the version of record. For permission to reproduce any abstract, contact the ARVO Office at arvo@arvo.org . 121 Ocular Fibrosis: Myofibroblast to Therapeutics - Minisymposium Sunday, May 05, 2013 10:30 AM-12:15 PM TCC 303 Minisymposium Program #/Board # Range: 388-392 Organizing Section: Cornea Contributing Section(s): Anatomy/Pathology, Glaucoma, Lens, Retina Program Number: 388 Presentation Time: 10:30 AM - 10:50 AM Fibrosis - Keeping an Eye on the Myofibroblast Boris Hinz. University of Toronto, Toronto, ON, Canada. Commercial Relationships: Boris Hinz, None Program Number: 389 Presentation Time: 10:50 AM - 11:05 AM Fibrosis in Glaucoma: Role in Pathologenesis and Surgical Failure Colm J. O'Brien. Ophthalmology, Mater Misericordiae Univ Hospital, Dublin, Ireland. Commercial Relationships: Colm J. O'Brien, None Program Number: 390 Presentation Time: 11:05 AM - 11:20 AM Prevention of Keratocyte-Myofibroblast Conversion by Targeting the Matrix-Cytokine System Shizuya Saika. Ophthalmology, Wakayama Medical University, Wakayama, Japan. Commercial Relationships: Shizuya Saika, None Program Number: 391 Presentation Time: 11:20 AM - 11:35 AM Fibrosis in the lens: Regulation of TGFß-induced lens Epithelial to Mesenchymal Transition (EMT) leading to cataract Frank J. Lovicu. Anatomy & Histology - F13, University of Sydney, Sydney, NSW, Australia. Commercial Relationships: Frank J. Lovicu, None Program Number: 392 Presentation Time: 11:35 AM - 11:50 AM Regulation of Retinal and Subretinal Fibrosis David R. Hinton. Pathology, Keck School of Medicine USC, Los Angeles, CA. Commercial Relationships: David R. Hinton, RPT (I), RPT (P) 126 Contact Lens I Sunday, May 05, 2013 10:30 AM-12:15 PM Exhibit Hall Poster Session Program #/Board # Range: 487-523/B0124-B0160 Organizing Section: Cornea Program Number: 487 Poster Board Number: B0124 Presentation Time: 10:30 AM - 12:15 PM Evaluation of Surface Water Characteristics of Novel Daily Disposable Contact Lenses Using Refractive Index Shifts after Wear Jeffery Schafer, Robert B. Steffen. Bausch & Lomb, Rochester, NY. Purpose: Several novel daily disposable contact lenses have been introduced with unique water characteristics. Nesofilcon A lenses are described as having 78% water, the same water content as the cornea throughout the lens matrix. Delefilcon A lenses are described as having a surface water content of 80% and a bulk water content of 33%. The delefilcon A high water content at the surface is reported to be the result of a surface modification using a copolymer of polyamidoamine and poly(acrylamide-acrylic acid) which is highly anionic (negative charge). During wear, these high water materials are exposed to air and tear components that may change the properties of the lenses. The objective of this study was to investigate surface water characteristics using refractive index shifts after wear with delfilcon A lenses compared to nesofilcon A and etafilcon A lenses. Methods: Twenty subjects wore each of the three lens types in a randomly determined order for 15 minutes. The worn lenses for each subject were measured for surface refractive index on the Metricon M-2010 Prism Coupler. To establish baseline refractive index values, unworn lenses of each type were also measured for refractive index directly from the package. Results: The mean changes in refractive index (unworn - worn) were 0.006 for nesofilcon A lenses, 0.012 for etafilcon A lenses and 0.093 for delefilcon A lenses. With the highly accurate measurement capability of the Metricon instrument, (routine refractive index accuracy of ± 0.00053 and standard deviation from 0.0008 to 0.0046), the difference between unworn and worn average values were statistically significant for each lens, p<0.0001. Conclusions: Lenses with higher surface water content have a surface refractive index closer to the refractive index of water (1.33), while lenses with lower surface water content will have a higher refractive index. The refractive index is typically 1.46-1.48 for a 20% water lens and 1.37-1.38 for a 75% water lens. The results of this study show a change in mean surface refractive index for the delefilcon A lenses from 1.34, typical of >80% water, to 1.43, typical of a 33% water, following just 15 minutes of wear due to a change in the water content at the surface. There was no change in refractive index at the surface following lens wear for either the 78% water nesofilcon A lenses or the 58% water etafilcon A lenses. Commercial Relationships: Jeffery Schafer, Bausch & Lomb (E); Robert B. Steffen, Bausch + Lomb (E) Program Number: 488 Poster Board Number: B0125 Presentation Time: 10:30 AM - 12:15 PM Effect of Protein Adsorption on Dewetting and Corneal cell adhesion on Contact Lenses Saad Bhamla, Claire M. Elkins, David Bergsman, Gerald G. Fuller. Chemical Engineering, Stanford University, Stanford, CA. Purpose: We discuss the influence of lysozyme adsorption on contact lenses using two different experimental approaches - dewetting on lenses and adhesion of corneal cells to a contact lens substrate. Methods: Dewetting is studied by stretching a contact lens flat on an elevation stage built on a miniature Langmuir trough. By raising the stage, the lens surface captures a sessile droplet coated with DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine) at surface pressure of the eye (20-25 mN/m). Liquid is slowly drained and dewetting dynamics captured using a CCD camera. Corneal cell adhesion is studied using an apparatus developed in the Fuller lab. A contact lens is descended upon a cultured monolayer of live epithelial cells. After a waiting period, the contact lens is sheared laterally relative to the bottom plate, subjecting the cells to a controlled strain while a force transducer measures the applied stress. Our rheometer-based design allows precise control over the strain down to 0.001 strain units, as well as highly sensitive measurement of the applied stress. The entire apparatus is mounted on a DIC microscope, allowing live cell imaging. Results: Two commercial lenses are tested for both experimental