Downloaded from www.microbiologyresearch.org by IP: 23.22.250.46 On: Sun, 07 Feb 2016 10:06:35 Reclassification of Staphylococcus jettensis De Bel et al. 2013 as Staphylococcus petrasii subsp. jettensis subsp. nov. and emended description of Staphylococcus petrasii Pantucek et al. 2013 Annelies De Bel, 1 Pavel S ˇ vec, 2 Petr Petra ´s ˇ, 3 Ivo Sedla ´c ˇek, 2 Roman Pantu ˚c ˇek, 4 Fedoua Echahidi, 1 Denis Pie ´ rard 1 and Peter Vandamme 5 Correspondence Annelies De Bel Annelies.DeBel@uzbrussel.be 1 Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel (VUB), Laarbeeklaan 101, 1090 Brussels, Belgium 2 Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, Bld. A25, 625 00 Brno, Czech Republic 3 National Reference Laboratory for Staphylococci, National Institute of Public Health, S ˇ roba ´ rova 48, 100 42 Praha 10, Czech Republic 4 Division of Genetics and Molecular Biology, Department of Experimental Biology, Faculty of Science, Masaryk University, Kotla ´r ˇska ´ 2, 611 37 Brno, Czech Republic 5 Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, K.L. Ledeganckstraat 35, 9000 Ghent, Belgium The type and clinical strains of two recently described coagulase-negative species of the genus Staphylococcus, Staphylococcus petrasii and Staphylococcus jettensis, were compared using dnaJ, tuf, gap, hsp60 and rpoB gene sequences, DNA–DNA hybridization, ribotyping, repetitive sequence-based PCR fingerprinting and extensive biochemical characterization. Based on the results, the species description of S. petrasii has been emended and S. jettensis should be reclassified as a novel subspecies within S. petrasii for which the name Staphylococcus petrasii subsp. jettensis subsp. nov. is proposed. The type strain is SEQ110 T (5LMG 26879 T 5CCUG 62657 T 5DSM 26618 T 5CCM 8494 T ). Recently two novel coagulase-negative species of the genus Staphylococcus were described: Staphylococcus petrasii (Pantu ˚c ˇek et al., 2013a) and Staphylococcus jettensis (De Bel et al., 2013). The description of the former was based on 13 human clinical isolates. Genotypic and phenotypic analyses revealed two closely related subspecies that were named Staphylococcus petrasii subsp. petrasii (9 isolates) and Staphylococcus petrasii subsp. croceilyticus (4 isolates). A few months later, the name S. jettensis was proposed to accommodate eight human clinical isolates including one, SEQ027, with an aberrant colony colour, biochemical characteristics and a unique position in the tuf-based phylogenetic tree (De Bel et al., 2013). In the absence of additional strains representing the same taxon, strain SEQ027 was not given separate taxonomic status (Christensen et al., 2001). Comparison of dnaJ, tuf, gap, hsp60 and rpoB gene sequences by means of the BioNumerics version 5.1 software package (Applied Maths) consistently revealed very high sequence similarity values among the type strains of S. petrasii subsp. petrasii (CCM 8418 T ) and S. jettensis (SEQ110 T ) on the one hand, and between S. petrasii subsp. croceilyticus CCM 8421 T and S. jettensis SEQ027, on the other. The similarity levels of the dnaJ, tuf, gap, hsp60 and rpoB gene sequences of S. petrasii subsp. petrasii CCM 8418 T and S. jettensis SEQ110 T were 99.5, 99.5, 99.7, 97.5 and 99.6 %, respectively; while the corresponding values for S. petrasii subsp. croceilyticus CCM 8421 T and S. jettensis SEQ027 were 99.9, 100, 100, 100 and 100 %. Hsp60 data for strains of the species S. jettensis were generated in the present study as described previously (Kwok & Chow, 2003); all other sequences were taken from the reports of Pantu ˚c ˇek et al. (2013a) and De Bel et al. The GenBank/EMBL/DDBJ accession numbers of the hsp60 sequences of the isolates are KJ540111 (MCC95747), KJ540112 (SEQ036), KJ540113 (SEQ110), KJ540114 (SEQ255), KJ540115 (SEQ256), KJ540116 (SEQ257), KJ540117 (SEQ258), and KJ540118 (SEQ259). Three supplementary tables and one supplementary figure are available with the online Supplementary Material. International Journal of Systematic and Evolutionary Microbiology (2014), 64, 4198–4201 DOI 10.1099/ijs.0.064758-0 4198 064758 G 2014 IUMS Printed in Great Britain