2S70 Klin Onkol 2012; 25(Suppl 2): 2S70– 2S77 Identiication and Characterisation of Pro-metastatic Targets, Pathways and Molecular Complexes Using a Toolbox of Proteomic Technologies Identiikace a charakterizace prometastatických cílů, drah a molekulárních komplexů s využitím proteomických technologií *Faktor J. 1 , *Dvorakova M. 1,2 , *Maryas J. 1 , *Struharova I. 1,2 , Bouchal P. 1,2 1 Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic 2 Regional Centre for Applied and Molecular Oncology, Masaryk Memorial Cancer Institute, Brno, Czech Republic *Authors contributed equally to this work. Summary Background: Cancer metastasis involves changes in signalling pathways, cell adhesion, migra- tion and invasiveness. Modern proteomic, mass spectrometry based techniques enable disco- very of new pro-metastatic proteins and their functional partners. Also, they might be involved in their functional characterisation and validation towards development of new diagnostic and therapeutic approaches. Aim: The aim of this communication is to describe current possibili- ties for proteomic techniques in the discovery and characterization of pro-metastatic targets. The NF-κB pathway is one of the players responsible for a number of pro-metastatic processes. The related proteins can be discovered using untargeted proteomic approaches by compa- ring proteomes with diferent metastatic potential. Stable isotope labelling based methods enable a parallel analysis of more tumour samples. The identiied pro-metastatic proteins can be characterised in relationship to cell migration, invasiveness and proliferation and in terms of their involvement in molecular complexes via protein-protein interactions. Advan- tages of the metabolic labelling based methods can be taken in these studies, the same ap- plies for characterisation of related surface proteins involved in cell adhesion, invasiveness and cell-to-cell communication. For clinical validation of pro-metastatic proteins in large sample cohorts, approaches of targeted proteomics based on selected reaction monitoring are becom- ing methods of choice. Conclusion: Current proteomics methods play an important role in the identiication of novel pro-metastatic proteins, pathways and molecular complexes, in their functional characterisation and validation towards diagnostic and therapeutic application. Key words metastasis – proteomics – tumor markers – cell migration – membrane proteins – signal tran- sduction – mass spectrometry – isotope labeling This work was supported by grant of Czech Science Foundation No. P304/10/0868, by European Regional Development Fund and the State Budget of the Czech Republic (RECAMO; CZ.1.05/2.1.00/03.0101) and by Large Infrastructure Project of Czech Ministry of Education, Youth and Sports (BBMRI_CZ: LM2010004). Práce byla podpořena grantem GA ČR P304/10/0868, projektem Velkých infrastruktur MŠMT (BBMRI_CZ LM2010004) a Evropským fondem pro regionální rozvoj a státním rozpočtem České republiky (OP VaVpI – RECAMO, CZ.1.05/2.1.00/03.0101). The authors declare they have no potential conflicts of interest concerning drugs, products, or services used in the study. Autoři deklarují, že v souvislosti s předmětem studie nemají žádné komerční zájmy. The Editorial Board declares that the manuscript met the ICMJE “uniform requirements” for biomedical papers. Redakční rada potvrzuje, že rukopis práce splnil ICMJE kritéria pro publikace zasílané do bi omedicínských časopisů.  Pavel Bouchal, Ph.D. Department of Biochemistry Faculty of Science Masaryk University Kotlarska 2 633 17 Brno Czech Republic e-mail: bouchal@chemi.muni.cz Submitted/Obdrženo: 5. 10. 2012 Accepted/Přijato: 5. 11. 2012