Polyacetylenic Compounds and Butanol Fraction from Bidens pilosa can Modulate the Differentiation of Helper T Cells and Prevent Autoimmune Diabetes in Non-Obese Diabetic Mice Shu-Lin Chang 14 Cicero Lee-Tian Chang 1 Yi-Ming Chiang 1 Rong-Hong Hsieh 2 Chii-Ruey Tzeng 3 Tung-Kung Wu 4 Huey-Kang Sytwu 5 Lie-Fen Shyur 1 Wen-Chin Yang 1 Affiliation 1 InstituteofBioAgriculturalSciences,AcademiaSinica,Taipei,Taiwan,R.O.C. 2 DepartmentofNutrition,TaipeiMedicalUniversity,Taipei,Taiwan,R.O.C. 3 DepartmentofObstetricsandGynecology,TaipeiMedicalUniversityHospital,Taipei,Taiwan,R.O.C. 4 DepartmentofBiologicalScienceandTechnology,NationalChiaoTungUniversity,Hsinchu,Taiwan,R.O.C. 5 DepartmentofMicrobiologyandImmunology,NationalDefenseMedicalCenter,Taipei,Taiwan,R.O.C. Correspondence Drs.Wen-ChinYang&Lie-FenShyur´InstituteofBioAgriculturalSciences´AcademiaSinica´ 128,AcademiaRoadSec.2´Nankang115´Taipei´Taiwan´R.O.C.´Fax:+886-2-26515120´ E-mail:wcyang@gate.sinica.edu.tw Received February9,2004´ Accepted April24,2004 Bibliography PlantaMed2004;70:1045±1051´GeorgThiemeVerlagKGStuttgart´NewYork DOI10.1055/s-2004-832645 ISSN0032-0943 Abstract CompellingevidencesuggeststhatinfiltratingCD4 + typeIhelper TTh1)cellsinthepancreaticisletsplayapivotalroleinthepro- gressionofdiabetesinnon-obesediabeticNOD)mice.Wede- monstrate in the present report that a butanol fraction of B. pilosa suppressedthedevelopmentofdiabetes,helpedmaintain levelsofbloodsugarandinsulininNODmiceinadose-depen- dentmannerandelevatedtheserumIgElevelsregulatedbyTh2 cytokinesinNODmice.Moreover,thebutanolfractioninhibited thedifferentiationofnaïvehelperTTh0)cellsintoTh1cellsbut enhancedtheirtransitionintotypeIIhelperTTh2)cellsusing an in vitro Tcelldifferentiationassay.Twopolyacetyleniccom- pounds, 2-b-D-glucopyranosyloxy-1-hydroxy-5E)-tridecene-7,9,11- triyne and 3-b-D-glucopyranosyloxy-1-hydroxy-6E)-tetradecene- 8,10,12-triyne, identified from the butanol fraction also prevented theonsetofdiabeteslikethebutanolfraction.Thelattercompound showedastrongeractivityforTcelldifferentiationthantheformer. Insummary,thebutanolfractionof B. pilosa anditspolyacetylenes canpreventdiabetesplausiblyviasuppressingthedifferentiationof Th0 cells into Th1 cells and promoting that of Th0 cells into Th2 cells. Key words T helper cells ´ Differentiation ´ non-obese diabetes ´ autoimmune diseases´ Bidens pilosa ´Asteraceae Abbreviations NOD: non-obesediabetic IDDM: insulin-dependentdiabetesmellitus PBS: phosphate-bufferedsaline PMA: phorbol12-myristate13-acetate PHA: phytohemagglutininfrom Phaseolus vulgaris IFN: interferon IL: interleukin LT: lymphotoxin TNF: tumornecrosisfactor HPLC: highperformanceliquidchromatography UV: ultraviolet FACS: fluorescenceactivatedcellsorter Th: helperTcells Th0: naïveTh Th1: typeITh Th2: typeIITh i. p.: intraperitoneal MTT: methylthiazoletetrazolium IC 50 : inhibitoryconcentration50% CC 50 : cytotoxicconcentration50% Original Paper 1045