http://informahealthcare.com/mdn ISSN: 1940-1736 (print), 1940-1744 (electronic) Mitochondrial DNA, Early Online: 1–2 ! 2015 Informa UK Ltd. DOI: 10.3109/19401736.2015.1018233 MITOGENOME ANNOUNCEMENT Complete mitochondrial genome sequence of Melipona scutellaris, a Brazilian stingless bee Ulisses de Padua Pereira 1,2 , Ana Maria Bonetti 1 , Luiz Ricardo Goulart 1 , Anderson Rodrigues dos Santos 3 , Guilherme Correa de Oliveira 4 , Sara Cuadros-Orellana 4 , and Carlos Ueira-Vieira 1 1 Institute of Genetics and Biochemistry, Federal University of Uberla ˆndia, Uberla ˆndia, MG, Brazil, 2 Department of Veterinary Preventive Medicine, Londrina State University, Londrina, PR, Brazil, 3 College of Computing, Federal University of Uberla ˆndia, Uberla ˆndia, MG, Brazil, and 4 Molecular and Cellular Pathology Laboratory, Rene ´ Rachou Research Center-Fiocruz, Belo Horizonte, MG, Brazil Abstract Melipona scutellaris is a Brazilian stingless bee species and a highly important native pollinator besides its use in rational rearing for honey production. In this study, we present the whole mitochondrial DNA sequence of M. scutellaris from a haploid male. The mitogenome has a size of 14,862 bp and harbors 13 protein-coding genes (PCGs), 2 rRNA genes and 21 tRNA genes. Keywords DNA sequencing, Melipona scutellaris, mitochondrial genome History Received 22 January 2015 Accepted 24 January 2015 Published online 23 February 2015 The Melipona scutellaris stingless bee is common in northeastern of Brazil where it is a very important native pollinator. The caste determination mechanism in Melipona genus is different from others bees. It is based on the interaction between environmental and genetic components (Kerr, 1950; Kerr & Nielsen, 1966). Meliponiculture is a rational rearing of those stingless bees and it is an important source of supplementary income for small farmers (Ueira-Vieira et al., 2013). So far, only one species from the genus (M. bicolor) had its whole mitochondrial DNA sequenced. Recently, our group had published a preliminary analysis of the mitochondrial DNA sequencing of the M. scutellaris (Silverio et al., 2014). Due the importance of the stingless bees, our goal was sequencing the whole DNA mitochondrial of the M. scutellaris. Five haploid male bees from the Meliponary at the Federal University of Uberla ˆndia – UFU (S 180 55 0 /W 450 17 0 ) were used for total DNA extraction. A 250-bp paired-end genomic Correspondence: Ueira-Vieira C, Institute of Genetics and Biochemistry, Federal University of Uberla ˆndia, Avenida Para ´ 1720, Campus Umuarama, Bloco 2E, Sala 230, 38400-902, Uberla ˆndia, MG, Brazil. Tel: +55 34 32182476. E-mail: ueira@ingeb.ufu.br Table 1. Annotation of mtDNA of M. scutellaris. Position Codon Gene From (bp) To (bp) Size (bp) Amino acid Start codon Stop codon Strand a tRNA Ile 100 160 67 H tRNA Ala 166 226 61 H tRNA Lys 227 295 67 L tRNA Met 306 375 70 H Nd2 443 1486 1044 347 ATA TAA H tRNA Cys 1425 1487 63 H tRNA Trp 1488 1552 65 H tRNA Tyr 1554 1620 67 L Cox1 1697 3244 1548 515 ATA TAA H tRNA Leu2 3248 3312 65 H Cox2 3313 3390 678 225 ATA TAA H tRNA Asp 4000 4065 66 H Atp8 4075 4233 159 52 ATA TAA H Atp6 4224 4907 684 227 ATG TAA H (continued ) Mitochondrial DNA Downloaded from informahealthcare.com by Universidade Estadual de Londrina on 02/23/15 For personal use only.