1
Proteomic study of neuron and astrocyte cultures from senescence-accelerated mouse
SAMP8 reveals degenerative changes
Cristina Díez-Vives
1
, Marina Gay
2
, Silvia García-Matas
1
, Francesc Comellas
3
, Montserrat
Carrascal
2
, Joaquín Abian
2
, Arantxa Ortega
4
, Rosa Cristòfol
1
, Coral Sanfeliu
1
1
Department of Brain Ischemia and Neurodegeneration, Institut d’Investigacions
Biomèdiques de Barcelona (IIBB), CSIC-IDIBAPS, Barcelona, Spain.
2
CSIC-UAB Proteomics Laboratory Facilities, Barcelona, Spain.
3
Department of Applied Mathematics IV, Universitat Politècnica de Catalunya (UPC),
Barcelona, Spain.
4
Department of Pathology, University Hospital Vall d’Hebron (HUVH), Barcelona, Spain.
Corresponding author:
Coral Sanfeliu, PhD
Department of Brain Ischemia and Neurodegeneration
Institut d’Investigacions Biomèdiques de Barcelona (IIBB)
CSIC-IDIBAPS
Rosselló 161, 6
th
floor, 08036 Barcelona, Spain.
E-mail: coral.sanfeliu@iibb.csic.es
Phone: (+34) 933638338
Fax: (+34) 933638301
Abbreviations: AD, Alzheimer’s disease; Aldh2, aldehyde dehydrogenase mitochondrial;
CHAPS, 3-[(3-cholamidopropyl) dimethylammonio] propanesulfonate; Cox, cytochrome
oxidase; DMEM, Dulbecco’s Modified Eagle’s Medium; FBS, fetal bovine serum; HPI,
human protein interactome network; MALDI-TOF-MS, matrix-assisted laser
desorption/ionization-time of flight-mass spectrometry; MOWSE, molecular weight
search; PBS, phosphate buffered saline; PMF, peptide mass fingerprint; PP1,
serine/threonine-protein phosphatase type 1; Ppp1ca, Serine/threonine-protein phosphatase
PP1-alpha catalytic subunit; SAMP8, senescence-accelerated prone mouse strain 8;
SAMR1, senescence-accelerated resistant mouse strain 1; SDS-PAGE, sodium dodecyl
sulfate-polyacrylamide gel electrophoresis; SUMO, small ubiquitin-like modifier.