Cryopreservation techniques 89 Session 2. Cryopreservation techniques Cryopreservation of undifferentiated plant cells Poula J. Reinhoud, Frank Van Iren and Jan W. Kijne 91 Cryopreservation of banana embryogenic cell suspensions: an aid for genetic transformation Bart Panis, Hilde Schoofs, Serge Remy, László Sági and Rony Swennen 103 Cryopreservation of sugarcane embryogenic callus Marcos E. Martínez-Montero, María Teresa González-Arnao, C. Borroto- Nordelo, C. Puentes-Díaz and Florent Engelmann 110 Cryopreservation of pollen Leigh E. Towill and Christina Walters 115 Use of stored pollen for wide crosses in wheat haploid production Masanori Inagaki 130 Storage of pollens for long-term conservation of yam genetic resources Nyat Quat Ng and I.O. Daniel 136 Cryopreservation of recalcitrant zygotic embryos Patricia Berjak, Marieanne Walker, D.J. Mycock, J. Wesley-Smith, Paula Watt and N.W. Pammenter 140 Cryopreservation of zygotic embryos of tropical fruit trees – a study on Lansium domesticum and Baccaurea species M.N. Normah, G. Mainah and R. Saraswathy 156 Cryopreservation of coffee (Coffea arabica L.) seeds: toward a simplified protocol for routine use in coffee genebanks S. Dussert, Nathalie Chabrillange, Florent Engelmann, F. Anthony and S. Hamon 161 Cryopreservation of melon somatic embryos by desiccation method Kei Shimonishi, Masaya Ishikawa, Seiichi Suzuki and Katsuji Oosawa 167 Cryopreservation of oil-palm polyembryonic cultures Dominique Dumet, Florent Engelmann, Nathalie Chabrillange, S. Dussert and Y. Duval 172 Recent developments in cryopreservation of shoot apices of tropical species Hiroko Takagi 178 Cryopreservation of vegetatively propagated species (mainly mulberry) Takao Niino, Ivette Seguel and Toru Murayama 194 Genotype considerations in temperate fruit crop cryopreservation Barbara M. Reed 200 Cryopreservation of in vitro-grown meristems of potato ( Solanum tuberosum L.) by encapsulation-vitrification Dai Hirai and Akira Sakai 205 Cryopreservation of in vitro-cultured meristems of wasabi Toshikazu Matsumoto 212