Downloaded from www.microbiologyresearch.org by IP: 54.162.133.179 On: Sun, 21 Feb 2016 21:33:07 Terrimonas rubra sp. nov., isolated from a polluted farmland soil and emended description of the genus Terrimonas Jing Zhang, 1 Tao Gu, 1 Yu Zhou, 2,3 Jie He, 3 Liu-Qiang Zheng, 3 Wen-Jun Li, 3 Xing Huang 1 and Shun-Peng Li 1 Correspondence Xing Huang huangxing@njau.edu.cn Yu Zhou microbes@yahoo.cn 1 Key Laboratory for Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China 2 State Key Laboratory Breeding Base for Zhejiang Sustainable Plant Pest Control, Agricultural Ministry Key Laboratory for Pesticide Residue Detection, Zhejiang Province Key Laboratory for Food Safety, and Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, 310021, PR China 3 The Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PR China A salmon-red-pigmented bacterial strain, designated M-8 T , was isolated from a polluted farmland soil sample in China and was characterized in a taxonomic study using a polyphasic approach. Strain M-8 T was Gram-stain-negative, rod-shaped, non-motile and non-spore-forming. Growth occurred at 20–37 6C, at pH 5.0–10.0 and with 0–2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M-8 T belonged to the genus Terrimonas.16S rRNA gene sequence similarity values between strain M-8 T and the type strains of the three recognized species of the genus Terrimonas, Terrimonas ferruginea KACC 11310 T , Terrimonas aquatica LMG 24825 T and Terrimonas lutea KACC 13047 T , were 97.1, 96.3 and 95.3 %, respectively. The predominant respiratory quinone was menaquinone-7 (MK-7) and the major fatty acids were iso-C 15 : 0 , iso-C 17 : 0 3-OH and summed feature 3 (comprising C 16 : 1 v7c and/or C 16 : 1 v6c). The DNA G+C content of strain M-8 T was 47.0 mol%. On the basis of genotypic and phenotypic data, strain M-8 T is considered to represent a novel species of the genus Terrimonas, for which the name Terrimonas rubra sp. nov. is proposed. The type strain is M-8 T (5CCTCC AB 2010401 T 5KCTC 23299 T ). An emended description of the genus Terrimonas is also presented. The genus Terrimonas was proposed by Xie & Yokota (2006) with Terrimonas ferruginea as the type species. At the time of writing, the genus Terrimonas comprised three recog- nized species: Terrimonas aquatica, Terrimonas lutea and T. ferruginea (T. ferruginea was previously described as [Flavobacterium] ferrugineum) (Sheu et al., 2010; Xie & Yokota, 2006). Members of the genus Terrimonas are generally characterized as strictly aerobic, Gram-negative, non-motile, non-gliding and non-filamentous bacteria that are oxidase-positive and weakly catalase-positive. These bacteria usually contain iso-C 15 : 0 , iso-C 15 : 1 G, iso-C 17 : 0 3- OH and summed feature 3 as major fatty acids, menaqui- none-7 (MK-7) as the predominant respiratory quinone and have DNA G+C contents of 47.0–48.9 mol%. During a project to investigate bacterial diversity from an imazethapyr (a weedkiller)-polluted farmland soil in Shandong Province, China, a salmon-red-pigmented bac- terial strain, designated M-8 T , was isolated and studied using a polyphasic taxonomic approach. Initial 16S rRNA gene sequence analysis indicated that strain M-8 T was related most closely to T. ferruginea KACC 11310 T , T. aquatica LMG 24825 T and T. lutea KACC 13047 T (97.1, 96.3 and 95.3 % similarity, respectively). Strain M-8 T clustered closely with the above type strains, and the four strains formed a separate phylogenetic branch within the family Chitino- phagaceae. To determine the taxonomic and phylogenetic position of strain M-8 T , T. ferruginea KACC 11310 T , T. aquatica LMG 24825 T and T. lutea KACC 13047 T were selected for comparison of morphological, physiological, biochemical and chemotaxonomic characteristics; and DNA–DNA hybridizations were also performed between strain M-8 T and its two closest phylogenetic neighbours The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain M-8 T is JF803808. A supplementary table is available with the online version of this paper. International Journal of Systematic and Evolutionary Microbiology (2012), 62, 2593–2597 DOI 10.1099/ijs.0.036079-0 036079 G 2012 IUMS Printed in Great Britain 2593