_____________________________________________________________________________________________________ *Corresponding author: E-mail: rouf321@yahoo.com; American Chemical Science Journal 5(3): 224-237, 2015, Article no.ACSj.2015.022 ISSN: 2249-0205 SCIENCEDOMAIN international www.sciencedomain.org Development and Validation of Colorimetric and RP- HPLC Methods for the Determination of Formaldehyde in Cosmetics Md. Motiur Rahman 1 , Harun-or-Rashid 1 , Subrata Bhadra 1 and A. S. Shamsur Rouf 1* 1 Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh. Authors’ contributions This work was carried out in collaboration among all authors. Author MMR performed the analytical studies and wrote the first draft of the manuscript. Author SB designed the study, wrote the protocol, managed the analyses of the study and wrote the final draft of the manuscript. Author HOR managed the literature searches and author ASSR supervised the overall study. All authors read and approved the final manuscript. Article Information DOI: 10.9734/ACSj/2015/12385 Editor(s): (1) Hsing-Lin Wang, Chemistry Division, Los Alamos National Laboratory, USA. (2) Sang Hak Lee, Department of Chemistry, Kyungpook National University, Daegu, 702-701, Korea. Reviewers: (1) Anonymous, University of Mysore, India. (2) Anonymous, Rio de Janeiro State University, Brazil. (3) Anonymous, Damascus University, Syria. (4) Anonymous, Hanyang University, Korea. (5) Anonymous, Hanyang University, Republic of Korea. (6) Mohamed Z.M. Salem, Forestry and Wood Technology Department, Faculty of Agriculture (EL–Shatby), Alexandria University, Egypt. Complete Peer review History: http://www.sciencedomain.org/review-history.php?iid=862&id=16&aid=7110 Received 29 th June 2014 Accepted 20 th October 2014 Published 8 th December 2014 ABSTRACT Aims: To develop and validate two assay methods for the determination of total and free formaldehyde content in cosmetics by Colorimetric and High Performance Liquid Chromatographic (HPLC) methods, respectively. Study Design: Colorimetric and HPLC methods. Place and Duration of Study: Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh; between February 2012 and June 2013. Short Research Article