S268 Abstracts / Toxicology Letters 205S (2011) S180–S300 P2286 Cr, Ni and Al dietary intake in children and teenagers in the Canary Islands (Spain): A total diet study C. Rubio Armendáriz * , D. González-Weller, A.J. Gutiérrez Fernández, C. Revert Gironés, A. Hardisson Toxicología, Universidad de La Laguna, La Laguna, Spain Purpose: Dietary intake is the main source of exposure to Chromium (Cr), Nickel (Ni) and Aluminum (Al) for human beings. Therefore, our objectives have been: - To quantify the concentra- tion of Cr, Ni and Al in 22 food groups. - To estimate the total dietary intakes of these metals in children (6–10 years) and teenagers (11–17 years) living in the Canary Islands. Material and methods: A total of 440 analyzed samples were analyzed (20 samples per food group). Cr and Ni were determined by Atomic Absorption Spectrometry (AAS) with a Graphite furnace (GFAAS). Al was analyzed by Optical Emission Spectrometry with Inductively Coupled Plasma (ICP-OES). Results and conclusions: Cr intake in children (age 6–10) is 0.087 mg/day for boys and 0.085 mg/day for girls. For teenagers (age 11–17), Cr intake is 0.106 mg/day for boys and 0.089 mg/day for girls. In children aged 6–10 years, Ni intake is estimated to be 0.092 and 0.089 mg/day for boys and girls, respectively. For teenagers (age 11–17) total Ni dietary is estimated to be 0.107 and 0.090 mg/day for males and females, respectively. In boys aged 6–10 years, Al intake is estimated in 8.356 mg/day, while in girls the same age is 8.575 mg/day. Teenagers (11–17 years) present and Al intake of 10.045 and 8.340 mg/day, respectively. This dietary intake data for trace and ultratrace metals will help regulatory agencies to deter- mine safe maximum/tolerable intakes. doi:10.1016/j.toxlet.2011.05.910 P2287 Occupational risk in veterinary oncology B.F. Sao Braz 1,* , J. Palminha 2 , G. Vicente 2 1 Pharmacology And Toxicology, Faculdade de Medicina Veterinária/CIISA, Lisboa, Portugal, 2 Hospital Escolar, FAculdade de Medicina Veterinária, Lisboa, Portugal Introduction: The use of cytotoxic drugs as antineoplastic agents and immunosuppressive treatment is expanding in veteri- nary medicine. Consequently the veterinarian, the technician, the cleaner, and the owner and his family are more frequently exposed to those drugs. Considering mutagenic, carcinogenic and terato- genic properties and other adverse effects of cytotoxic drugs is necessary to improve and follow protection rules to decrease risk of exposure to them. The European College of Veterinary Internal Medicine (ECVIM) provides to veterinary professionals a guideline concerning prevention occupational and environmental exposure to cytotoxic drugs in veterinary medicine. Purpose: To know if Portuguese veterinarians respect and prac- tice protection rules, to prevent cytotoxic drugs exposure, a query was elaborated and sent by email to Veterinary Clinics and Hos- pitals all over the country. Questions were related with protection measures observed during preparation, handling, administration and elimination of cytotoxic drugs, and also with animal owner’s information about hazards. Anonymous answers were received from 33 Clinics and 11 Hospitals. The results obtained allow concluding that protection measures recommended in the ECVIM guideline are not completely followed and so a high occupational risk is presented by veterinary profes- sionals. Considering these results, some measures are proposed by the authors to improve safe use and handling of cytotoxic drugs. Urgent major measures are: personal protection equipment for all exposure phases, cytotoxic spill kit for cleaning of spilled drugs, preparation of drugs inside a biological flow cabinet, administration of drug using specific equipments and materials, written informa- tion on the potential hazards provided to animal owners. doi:10.1016/j.toxlet.2011.05.911 P2288 Comparing the safety margins in the European indicative occupational exposure limits and the derived no-effect levels under reach L. Schenk 1,* , G. Johanson 2 1 Division of Philosophy, Royal Institute of Technology, Stockholm, Sweden, 2 Institute of Environmental Medicine, Karolinska Institute, Stockholm, Sweden The new European Union (EU) REACH legislation requires Derived No-Effect Levels (DNEL) to be calculated for substances produced in quantities above 10 tonnes/year. Workers are one pop- ulation that these DNELs are to protect. Meanwhile, the setting of occupational exposure limits (OEL) continues both at the mem- ber state and the EU level. Health-based OELs are proposed by the Commission’s Scientific Committee on OELs (SCOEL) and eventually result in Indicative OEL Values (IOELV) in EU Directives. Accord- ing to REACH, IOELVs may under some circumstances be used as worker-DNELs. On the other hand, worker-DNELs will be derived for several thousand substances, far more than the roughly 100 substances for which IOELVs have been established. Thus, the pro- cedure to set health-based OELs may become influential on that of DNELs and vice versa. This study presents a comparison of the safety margins of 88 SCOEL recommendations with those of the corre- sponding worker-DNELs, derived according to the REACH guidance document. Overall, the REACH safety margins were approximately six times higher than those derived from the SCOEL documentation but varied widely with REACH/SCOEL ratios ranging by two orders of magnitude, from 0.3 to 58. It was also found that the REACH guid- ance document, although encompassing detailed advice on many issues, including default assessment factors for species and route extrapolation, gives no quantitative guidance on when and how to depart from defaults. doi:10.1016/j.toxlet.2011.05.912 P2289 Mercury in food and environment C. Siegers 1,* , H.D. Reusch 2 , X. Hao 2 , J.M. Traeder 1 1 Department of Experimental and Clinical Pharmacology and Toxicology, Medical University of Lübeck, Lübeck, Germany, 2 Angewandte Naturwissenschaften, University of applied Sience, Lübeck, Germany Elemental mercury is volatile under room temperature. Inges- tion by human is almost of no harm but inhalation into ling then into blood can cause various symptoms and severest can be death. But in normal life, the chance of exposure to this kind of accident is relatively small for non-occupational people. The most danger- ous and toxic pollutant for human is methylmercury for the reason