Downloaded from www.microbiologyresearch.org by IP: 54.162.190.106 On: Mon, 29 Feb 2016 18:35:48 Cellulophaga pacifica sp. nov. Olga I. Nedashkovskaya, 1 Makoto Suzuki, 2 Anatoly M. Lysenko, 3 Cindy Snauwaert, 4 Marc Vancanneyt, 4 Jean Swings, 4 Mikhail V. Vysotskii 5 and Valery V. Mikhailov 1 Correspondence Olga I. Nedashkovskaya olganedashkovska@piboc.dvo.ru or olganedashkovska@yahoo.com 1 Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 Let Vladivostoku 159, 690022, Vladivostok, Russia 2 Tokyo Research Laboratories, Kyowa Hakko Kogyo Company Ltd, 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan 3 Institute of Microbiology of the Russian Academy of Sciences, Pr. 60 let October 7/2, Moscow, 117811, Russia 4 BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B 9000 Ghent, Belgium 5 Institute of Marine Biology of the Far-Eastern Branch of the Russian Academy of Sciences, Pal’chevskogo St 17, 690032, Vladivostok, Russia Three marine, heterotrophic, aerobic, agarolytic, pigmented and gliding bacteria were isolated in June 2000 from a sea water sample that was collected in the Gulf of Peter the Great, Sea of Japan, and analysed in a polyphasic taxonomic study. 16S rDNA sequence analysis indicated that strains KMM 3664 T , KMM 3669 and KMM 3915 were members of the family Flavobacteriaceae. Based on phenotypic, chemotaxonomic, genotypic and phylogenetic data, the isolates were classified in the genus Cellulophaga as members of a novel species, Cellulophaga pacifica sp. nov. The type strain is KMM 3664 T (=JCM 11735 T =LMG 21938 T ). The genus Cellulophaga was proposed by Johansen et al. (1999) as a separate branch in the family Flavobacteriaceae, to comprise three species: Cellulophaga baltica and Cellulo- phaga fucicola, two agarolytic, yellow–orange-pigmented bacteria associated with the brown alga Fucus serratus, and Cellulophaga lytica, a common representative of coastal microbial communities that was formerly misclassified as [Cytophaga] lytica (Lewin, 1969; Reichenbach, 1989). The latter species was assigned as the type species of the genus. A fourth member of the genus, Cellulophaga algicola, which originated from the surface of algal species from Antarctic marine coasts and sea ice, was described relatively recently (Bowman, 2000). This author also reclassified [Cytophaga] uliginosa (Reichenbach, 1989), formerly Flavobacterium uliginosum (ZoBell & Upham, 1944), in the genus Cellulo- phaga as Cellulophaga uliginosa. Recently, C. uliginosa has been transferred to a novel genus, Zobellia, based on DNA G+C content, maximum growth temperature, presence of flexirubin and phylogenetic position (Barbeyron et al., 2001). Three gliding, agarolytic, strictly aerobic, Gram-negative and yellow-pigmented bacterial strains were isolated from a sea water sample that was collected in the Sea of Japan, Pacific Ocean. Phenotypic, chemotaxonomic and genotypic characteristics assigned these bacteria to the family Flavo- bacteriaceae. Phylogenetic and phenotypic data indicated that the unknown organisms comprised a distinct species within the genus Cellulophaga. The name Cellulophaga pacifica sp. nov. is proposed for these sea water isolates; the type strain is KMM 3664 T (=JCM 11735 T =LMG 21938 T ). Strains KMM 3664 T , KMM 3669 and KMM 3915 were isolated from a sea water sample that was collected during June 2000 in Amursky Bay, Gulf of Peter the Great, Sea of Japan, Pacific Ocean, from a depth of 5 m (salinity, 33 %; temperature, 15 uC). After primary isolation and purifica- tion, strains were cultivated at 28 u C on marine agar 2216 (Difco) and stored at 280 u C in marine broth (Difco) supplemented with 20 % (v/v) glycerol. The bacteria isolated in this study and reference strains are shown in Table 1. Abbreviations: ACAM, Australian Collection of Antarctic Microorganisms, University of Tasmania, Hobart, Tasmania, Australia; ATCC, American Type Culture Collection, Manassas, VA, USA; JCM, Japan Collection of Microorganisms, Institute of Physical and Chemical Research (RIKEN), Wako, Japan; KMM, Collection of Marine Microorganisms of the Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia; LMG, BCCM/LMG Bacteria Collection, Laboratorium Microbiologie, Universiteit Gent, Belgium; NN, Enzyme Research, Novo Nordisk A/S, Bagsvaerd, Denmark. Published online ahead of print on 31 October 2003 as DOI 10.1099/ ijs.0.02737-0. The GenBank/EMBL/DDBJ accession numbers for the 16S rDNA sequences of Cellulophaga pacifica KMM 3664 T , KMM 3669 and KMM 3915 are AB100840, AB100841 and AB100842, respectively. 02737 G 2004 IUMS Printed in Great Britain 609 International Journal of Systematic and Evolutionary Microbiology (2004), 54, 609–613 DOI 10.1099/ijs.0.02737-0