UNCORRECTED PROOF 1 Research review paper 2 Q1 Biocatalysis and biotransformation in Brazil: An overview 3 Q2 Willian G. Birolli a,1 , Irlon M. Ferreira a,1 , Natália Alvarenga a,1 , Darlisson A. dos Santos a,1 , Iara L. de Matos a,1 , 4 João V. Comasseto b,c , André L.M. Porto a,1 Q3 5 a Laboratório de Química Orgânica e Biocatálise, Instituto de Química de São Carlos, Universidade de São Paulo, Av. João Dagnone, 1100, J. Santa Angelina, 13563-120, São Carlos, SP, Brazil 6 b Instituto de Química, Departamento de Química Fundamental,Universidade de São Paulo, Av. Prof. Lineu Prestes, 748, 05508-000 São Paulo, SP, Brazil 7 c Instituto de Ciências Ambientais, Químicas e Farmacêuticas,Universidade Federal de São Paulo, Av. Prof. Artur Riedel, 275, 09972-270 Diadema, SP, Brazil Q4 abstract 8 article info 9 10 Available online xxxx 11 Keywords: 12 Brazilian microorganisms 13 Biotransformation 14 Biocatalysis 15 Lipases 16 This review presents the recent research in biocatalysis and biotransformations in Brazil. Several substrates were 17 biotransformed by fungi, bacteria and plants. Biocatalytic deracemization of secondary alcohols, oxidation of 18 sulfides, sp 3 CH hydroxylation and biocatalytic epoxidation of alkenes were described. Chemo-enzymatic resolu- 19 tion of racemic alcohols and amines were carried out with lipases using several substrates containing hetero- 20 atoms such as silicon, boron, selenium and tellurium. Biotransformation of nitriles by marine-fungi, hydrolysis 21 of epoxides by microorganisms of Brazilian origin and biooxidation of natural products were reported. Enzymatic 22 reactions under microwave irradiation, continuous flow, and enzymatic assays using fluorescent probes were 23 reported. 24 © 2015 Published by Elsevier Inc. 25 26 27 28 29 30 Contents 31 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0 32 Biocatalytic reactions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0 33 Reduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0 34 Reduction by Saccharomyces cerevisiae . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0 35 Reduction by other microorganisms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0 36 Reduction by whole cells of plant and algae . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0 37 Reduction of organic compounds containing heteroatoms (sulfur, selenium and silicon) by whole cells of microorganisms and vegetables . . 0 38 Biocatalytic oxidation of sulfides by microorganisms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0 39 Biocatalytic sp 3 C-H hydroxylation with whole cells of microorganisms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0 40 Biocatalytic kinetic resolution by lipases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0 41 Biocatalytic deracemization of secondary alcohols using whole cells of microorganisms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0 42 Biocatalytic epoxidation of alkenes by lipases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0 43 Biocatalytic reactions in organic compounds containing heteroatoms (silicon, boron, selenium and tellurium) . . . . . . . . . . . . . . . . . . . . 0 44 Miscellaneous biotransformation by microorganisms of Brazilian origin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0 45 Biotransformation of nitriles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0 46 Biotransformation of epoxides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0 47 Biotransformation of natural products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0 48 Enzymatic assays using fluorescent probes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0 49 Conclusion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0 50 Uncited references . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0 51 Acknowledgments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0 52 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0 53 54 Introduction 55 The importance of biocatalysis in organic synthesis, especially in 56 the industrial synthesis of pharmaceuticals, is well established and 57 Q5 dismisses further comments (Aldridge, 2013; Patel, 2008). Biotechnology Advances xxx (2015) xxx–xxx E-mail addresses: jvcomass@iq.usp.br (J.V. Comasseto), almporto@iqsc.usp.br (A.L.M. Porto). 1 Tel.: +55 16 3373 8103; fax: +55 16 3373 9952. JBA-06894; No of Pages 30 http://dx.doi.org/10.1016/j.biotechadv.2015.02.001 0734-9750/© 2015 Published by Elsevier Inc. Contents lists available at ScienceDirect Biotechnology Advances journal homepage: www.elsevier.com/locate/biotechadv Please cite this article as: Birolli WG, et al, Biocatalysis and biotransformation in Brazil: An overview, Biotechnol Adv (2015), http://dx.doi.org/ 10.1016/j.biotechadv.2015.02.001