Please cite this article in press as: M.d.C. Montero-Calasanz, et al., Chryseobacterium oleae sp. nov., an efficient plant growth promoting bacterium in the rooting induction of olive tree (Olea europaea L.) cuttings and emended descriptions of the genus. . . , Syst. Appl. Microbiol. (2014), http://dx.doi.org/10.1016/j.syapm.2014.04.004 ARTICLE IN PRESS G Model SYAPM-25619; No. of Pages 9 Systematic and Applied Microbiology xxx (2014) xxx–xxx Contents lists available at ScienceDirect Systematic and Applied Microbiology j ourna l h omepage: www.elsevier.de/syapm Short communication Chryseobacterium oleae sp. nov., an efficient plant growth promoting bacterium in the rooting induction of olive tree (Olea europaea L.) cuttings and emended descriptions of the genus Chryseobacterium, C. daecheongense, C. gambrini, C. gleum, C. joostei, C. jejuense, C. luteum, C. shigense, C. taiwanense, C. ureilyticum and C. vrystaatense Maria del Carmen Montero-Calasanz a,b,∗∗ , Markus Göker a , Manfred Rohde c , Cathrin Spröer a , Peter Schumann a , Hans-Jürgen Busse d , Michael Schmid e , Hans-Peter Klenk a , Brian J. Tindall a,∗ , Maria Camacho b a Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany b IFAPA-Instituto de Investigación y Formación Agraria y Pesquera, Centro Las Torres-Tomejil, Ctra, Sevilla-Cazalla de la Sierra, Km 12.2, Alcalá del Río, 41200 Sevilla, Spain c HZI – Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124 Braunschweig, Germany d Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria e Research Unit Microbe-Plant Interactions, Helmholtz Zentrum München, Ingolstädter Landstraße 1, 85764 Neuherberg, Germany a r t i c l e i n f o Article history: Received 5 November 2013 Received in revised form 21 February 2014 Accepted 22 April 2014 Keywords: Bacteriodetes Flavobacteriaceae Taxonomy PGPB Indole-3-acetic acid a b s t r a c t A novel non-motile, Gram-staining-negative, yellow-pigmented bacterium, designated CT348 T , isolated from the ectorhizosphere of an organic olive tree in Spain and characterised as an efficient plant growth promoting bacterium, was investigated to determine its taxonomic status. The isolate grew best in a temperature range of 5–35 ◦ C, at pH 5.0–8.0 and with 0–1% (w/v) NaCl. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Chryseobacterium. The DNA G + C content of the novel strain was 38.2 mol%. The strain contained a polyamine pattern with sym-homospermidine as the major compound and produced flexirubin-type pigments. MK-6 was the dominant menaquinone and the major cellular fatty acids were iso-C 15:0 , C 17:1 9c, iso-C 17:0 3-OH and iso-C 15:0 2-OH. The main polar lipids were phosphatidylethanolamine and several unidentified lipids and aminolipids. The 16S rRNA gene showed 92.2–97.8% sequence identity with the members of the genus Chyseobacterium. Based on the phenotypic traits and DNA–DNA hybridizations with the type strains of the most closely related species, the isolate is shown to represent a novel species, Chyseobacterium oleae, type strain CT348 T (=DSM 25575 =CCUG 63020). Emended descriptions of the genus Chryseobacterium and C. daecheongense, C. gambrini, C. gleum, C. joostei, C. jejuense, C. luteum, C. shigense, C. taiwanense, C. ureilyticum and C. vrystaatense are also proposed. © 2014 Elsevier GmbH. All rights reserved. The genus Chryseobacterium [58] whose type species is Chryseobacterium gleum [21,58] is included within the family Flavobacteriaceae (emended by Bernardet et al. [4]), the main bacterial lineage in the phylum Bacteroidetes. At the time of writing 76 species with validly published names were considered to be ∗ Corresponding author. Tel.: +49 0531 26 16 224; fax: +49 0531 26 16 418. ∗∗ Corresponding author. Tel.: +49 0531 26 16 210; fax: +49 0531 26 16 418. E-mail addresses: maria.montero-calasanz@dsmz.de (M.d.C. Montero-Calasanz), bti@dsmz.de (B.J. Tindall). members of the genus Chryseobacterium based on the interpre- tation of the list of all validly published names within this genus according to the “List of Prokaryotic Names with Standing in Nomenclature” (http://www.bacterio.net/). Members of the genus Chryseobacterium can be found in a wide variety of environments, including freshwater [50], soil [32], rhizosphere [9], phyllosphere [3], sludge [43], fish [68–70], midgut of insects [26], raw dairy products [19], raw chicken [11], faeces of millipede [27], industrial plants [20] and clinical samples [67]. Based on previous studies, it is known that members of the genus Chryseobacterium are considered to be an important bacterial http://dx.doi.org/10.1016/j.syapm.2014.04.004 0723-2020/© 2014 Elsevier GmbH. All rights reserved.