400 MAIN ©1996-2013 All Rights Reserved. Online Journal of Bioinformatics . You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJB publications. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page. OJBTM Online Journal of Bioinformatics © Volume 13(3):400-417, 2012. Amino acid sequences based phylogenetic and motif assessment of lipases from different organisms Santosh Kumar Yadav 1 , Amit Kumar Dubey 2 , Sangeeta Yadav 2 , Deepali Bisht 1 , Nandan Singh Darmwal 1 and Dinesh Yadav 2 * 1 Department of Microbiology (Centre of Excellence), Dr. R.M. L. Avadh University, Faizabad, Uttar Pradesh- 224 001, INDIA 2 Department of Biotechnology, D.D.U Gorakhpur University, Gorakhpur, Uttar Pradesh, 273 009, INDIA ABSTRACT Yadav SK, Dubey AK, Yadav S, Bisht D, Darmwal NS, Yadav D., Amino acid sequences based phylogenetic and motif assessment of lipases from different organisms, Online J Bioinform., 13(3):400-417, 2012. Bacterial, fungal, plant and animal lipases amino acid sequences retrieved from different databases were analyzed for homology search, multiple sequence alignment, phylogenetic and motif assessment using different bioinformatics tools. The multiple sequence alignment revealed different conserved stretches of amino acids exclusively for each groups along with a highly conserved glycine residue for plant and animal lipases. The phylogenetic tree constructed for different groups uniformly showed two major clusters divided into several sub-clusters based on the closeness of the lipases protein sequences irrespective of the source organisms. The 64 bacterial lipases protein sequences were distributed into two main clusters with various sub-clusters revealing the rich diversity of lipases among diverse bacterial strains. The fungal lipase protein sequences based phylogenetic tree revealed two major clusters for representing two important groups namely ascomycota and basidomycota with several sub-clusters. The Aspergillus species were placed closely in the cluster along with Penicillium species. The distributions of three conserved motifs were analyzed among these lipases. Bacterial lipases motifs represented α/β hydƌolase fold faŵily ǁhile fuŶgal, aŶiŵal aŶd plaŶt lipases ƌeǀealed lipase 3 faŵilies though all represented the same Clan 0028. The motif sequences of these lipases also showed five conserved amino acids residues GHSAG for bacterial, fungal and plant lipases though it was not observed in the case of animal lipases. Key Words: Lipases, Phylogenetic tree, Motif analysis, Bacterial, Plant, Fungal, Animal