~ 419 ~  Volume: 2, Issue: 5, 419-422 May 2015 www.allsubjectjournal.com e-ISSN: 2349-4182 p-ISSN: 2349-5979 Impact Factor: 3.762 Suresh K.Ghotekar S.N.Arts D.J.M.Comm. and B.N.S. Science College, Sangamner, Maharashtra, India Priyanka S.Vaidya S.N.Arts D.J.M.Comm. and B.N.S. Science College, Sangamner, Maharashtra, India Sachin N.Pande S.N.Arts D.J.M.Comm. and B.N.S. Science College, Sangamner, Maharashtra, India Sharad P.Pawar Savitribai Phule Pune University, Pune, India Correspondence: Suresh K.Ghotekar S.N.Arts D.J.M.Comm. and B.N.S. Science College, Sangamner, Maharashtra, India Synthesis of silver nanoparticle by using 3-methyl pyrazol 5-one (chemical reduction method) and its characterization Suresh K.Ghotekar, Priyanka S.Vaidya, Sachin N.Pande, Sharad P.Pawar Abstract The synthesis of metal nanoparticles is in vogue due to their applications in various fields. We synthesized nanoparticles by chemical reduction method. In this study, first we prepared reducing agent (3-methyl pyrazol-5-one) by chemical method and that reducing agent is then used for the preparation of silver nano particles. The characterization of silver nanoparticles was done by using UV-Visible spectrophotometer, FTIR, SEM and XRD. It was found that the growth of Escherchia coli and Klebsiella pneumoniae was inhibited by this synthesized silver nanoparticles. The average particle size of the silver nano particles calculated from XRD spectra is about 17.68 nm. Keywords: Nanoparticles, UV-Visible spectrophotometer, FTIR, SEM and XRD, Antibacterial activity Introduction The preparation and characterization of novel nano structures such as cubes, disks, plates, prisms, wires, rods and triangle etc. The various metal are used for the synthesis of nanoparticles such as Silver, Tin, Cadmium, Zinc, Copper and Gold have come a long way of various investigation in many laboratories and there uses in photography, catalysis, biological labeling, superconductors, photonics, sensor, antimicrobial and antibacterial activities etc. Chemical reduction is the most frequently applied method for the preparation of the stable silver nanoparticles. The chemical reduction methods are probably the most versatile, economical and easy to control the shape and size of nanoparticles. The 3-methyl pyrazol-5- one is a one of the heterocyclic compound and this compound shows therapeutic importance. Pyrazolone derivatives have been used as analgesics, antimicrobial agents, fungicides and hypoglycemic agents. Some of them have been tested as potential cardiovascular drugs. They inhibit the production of TNF- α and decreases level of pro-inflammatory cytokines and thereby reduce inflammation and prevent further tissue destruction diseases such as Rheumatoid Arthritis, Osteoarthritis and Crohns disease. It is believed that the redox chemistry of 3-methyl pyrazol-5-one plays an important role in the pharmacy and the silver nanoparticles may be useful. In this work we focus, for the first synthesis of 3-methyl pyrazol-5-one, synthesis of silver nanoparticles and the reduction of Ag + ions by 3-methyl pyrazol-5-one. 2. Experimental: All chemicals used in the present study are of high purity. 2.1. Synthesis of 3-methyl pyrazol-5-one 5.2 ml (0.04 mole) of ethyl acetoacetate was taken in 25 ml beaker and stirred magnetically during slow drop wise addition of a solution of 2 ml (0.04 mole) of NH 2 NH 2 .H 2 O (Hydrazine hydrate) in 3.5 ml ethanol. The temperature of the reaction mixture increased during reaction so that temperature was regulated at 55˚ C. The reaction mixture was cooled in an ice bath to complete the crystallization. After standing for some time for complete of crystallization, it was filtered and the solid was washed with cold alcohol, dried and used for next step. (Yield = 76.21% & melting point = 223˚ C) International Journal of Multidisciplinary Research and Development