Development of InDel markers linked to Fusarium wilt resistance in cabbage Hong-hao Lv • Li-mei Yang • Jun-gen Kang • Qing-biao Wang • Xiao-wu Wang • Zhi-yuan Fang • Yu-mei Liu • Mu Zhuang • Yang-yong Zhang • Yan Lin • Yu-hong Yang • Bing-yan Xie • Bo Liu • Ji-Sheng Liu Received: 30 January 2013 / Accepted: 10 July 2013 / Published online: 14 August 2013 Ó Springer Science+Business Media Dordrecht 2013 Abstract Cabbage Fusarium wilt (CFW) is a destructive disease causing great losses to cabbage (Brassica oleracea L. var. capitata L.) production worldwide. At present, there are few reports concern- ing molecular marker research on cabbage resistance to CFW. In this study, 160 double haploid (DH) lines were obtained from the F1 population of a 99–77 (highly resistant to CFW) 9 99–91 (highly suscepti- ble to CFW) cross. Insertion–deletion (InDel) markers were designed according to the reference genome sequence of cabbage and the whole-genome re- sequencing data of the two parents. A genetic map of chromosome C06 including seven InDel markers was constructed based on the DH population. Thus, FOC (resistance gene to Fusarium oxysporum f. sp. conglutinans) was located on chromosome C06 and two InDel markers out of the seven, M10 and A1, flanked the gene at 1.2 and 0.6 cM, respectively. Marker A1 revealed a significant consistency with the phenotype assay in the F2 population as well as in 40 inbred lines (96 and 82 %, respectively). This study lays the foundation for fine mapping and cloning of the FOC gene and for marker-assisted selection in cabbage resistance breeding. Keywords Brassica oleracea Á Fusarium wilt Á MAS (marker-assisted selection) Á FOC gene Á InDel marker Introduction Cabbage (Brassica oleracea L. var. capitata L.) is an important cruciferous vegetable that is grown world- wide. According to the report of the Food and Agriculture Organization of the United Nations (FAO), the world production of cabbage and other Brassicas in 2010 was 57,966,986 metric tons, almost half of which were grown in China. Cabbage Fusarium wilt (CFW) is a destructive disease which for many years has brought a decline in cabbage quality and large losses in cabbage yields all over the world. In the 1890s, CFW was first reported in the USA by Smith (1899). It was then found in Japan and several other countries in the following decades (Nomura et al. 1976). In recent years, CFW has been identified in several provinces in China (Li et al. 2003; Zhang et al. 2008; Lv et al. 2011). CFW is a typical soil-borne disease, with its pathogen remaining in the soil for years or even decades once it occurs. H. Lv Á L. Yang (&) Á Q. Wang Á X. Wang Á Z. Fang Á Y. Liu Á M. Zhuang Á Y. Zhang Á Y. Lin Á Y. Yang Á B. Xie Á B. Liu Á J.-S. Liu Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 12# ZhongGuanCun Nandajie, Beijing 100081, China e-mail: yanglm@mail.caas.net.cn; yanglimei@caas.cn J. Kang Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, 5# LanDianChang NanLu, Beijing 100081, China e-mail: kangjungen@nercv.org 123 Mol Breeding (2013) 32:961–967 DOI 10.1007/s11032-013-9925-x