Oestrogen regulates bone resorption and cytokine production in the maxillae of female mice Soraia Macari a , Letı ´cia F. Duffles a , Celso M. Queiroz-Junior a , Mila F.M. Madeira a , George J. Dias b , Mauro M. Teixeira c , Raphael E. Szawka d , Tarcı ´lia A. Silva a, * a Department of Oral Pathology and Surgery, Faculty of Dentistry, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil b Department of Anatomy, University of Otago, Dunedin, New Zealand c Department of Biochemistry and Immunology, Biological Science Institute, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil d Department of Physiology and Biophysics, Biological Science Institute, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil a r c h i v e s o f o r a l b i o l o g y 6 0 ( 2 0 1 5 ) 3 3 3 – 3 4 1 a r t i c l e i n f o Article history: Accepted 16 November 2014 Keywords: Estrogens Maxilla Alveolar bone loss Cytokine Osteoporosis a b s t r a c t Oestrogen plays major role in bone metabolism/remodelling. Despite of well-established effect of oestrogen deficiency on long bones, it remains unclear whether alveolar bone is affected. We aimed to determine the effect of oestrogen-deficiency in the alveolar bone microarchitecture. C57BL6/J and Balb/c mice were ovariectomized and implanted with oil- (OVX) or 17b-estradiol (E2)-containing (OVX + E2) capsules. Ovary-intact mice were used as controls. The dose of E2 replacement was selected based on trophic effects on the uterus and femur bone loss. As determined by maxillary alveolar bone MicroCT analysis, both C57BL6/J and Balb/c OVX mice displayed decreased trabecular thickness, bone density and bone volume, and increased trabecular separation at 15 and 30 days after ovariectomy. These effects were associated with a reduction of trabecular bone percentage and cortical thick- ness in the femur. A significant loss of alveolar bone crest was also associated with ovariectomy in both mice strains. The E2 replacement fully prevented ovariectomy-induced alterations in the alveolar and femoral bones. Moreover, TNF-a (tumour necrosis factor-a) levels and RANKL/OPG (receptor activator of NF-kB ligand/osteoprotegerin) ratio were increased in the maxilla after OVX, and these responses were also reversed by E2. In conclusion, oestrogen deficiency causes maxillary alveolar bone loss, which is similar to the effects found in the femur. The release of inflammatory molecules like TNF-a, RANKL and OPG is the potential mechanism to the decrease of bone quality and alveolar bone crest. # 2014 Elsevier Ltd. All rights reserved. * Corresponding author at: Departamento de Clı´nica, Patologia e Cirurgia Odontolo ´ gicas, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Av. Presidente Anto ˆ nio Carlos 6627, CEP 31.270-901, Belo Horizonte, Minas Gerais, Brazil. Tel.: +55 3134092478. E-mail address: tarcilia@ufmg.br (T.A. Silva). Available online at www.sciencedirect.com ScienceDirect journal homepage: http://www.elsevier.com/locate/aob http://dx.doi.org/10.1016/j.archoralbio.2014.11.010 0003–9969/# 2014 Elsevier Ltd. All rights reserved.