Anti-aggregation effects of processed yellow and white Garri Aqueous Filtrates on sickle cell Trait Haemoglobin. Keywords: Anti-aggregation, HbAS, processed yellow and white garri, aqueous filtrate. ABSTRACT: Aqueous filtrates of processed yellow and white garri {fried fermentation products of cassava (Manihot esculenta)} were investigated for their effects on the aggregation of sickle cell trait haemoglobin (HbAS), in vitro. Phytochemicals detected in the two filtrates included tannins, catechins, flavonoids, cyanogenic glycosides, β-carotene and thiocyanates. Biomolecules such as amino acids, monosaccharides and ρ-hydroxybenzoic acid (ρ-HBA) were also present. Saponins and cardiac glycosides were not detected in either of the filtrates. The processed white garri aqueous filtrate did not contain β-carotene. Palm oil did not contain cardiac glycosides. The amino acid with the highest (p<0.05) concentration in the palm oil was aspartic acid (6.29±0.03 g/100 g protein) whereas cysteine and methionine had the lowest (p<0.05) concentrations (0.56±0.08 and 0.57±0.04 g/100 g protein, respectively). Spectrophotometric method was used to investigate the aggregation of haemolysate HbAS molecules treated with sodium metabisulphite (Na 2 S 2 O 5 ) at λ max = 555 nm for 25 minutes. The present study showed that aqueous filtrate of processed yellow garri exhibited a significantly ( p<0.05) higher (82.91%) aggregation inhibitory effect than the aqueous filtrate of processed white garri which had 64.66%. 047-052 | JRBC | 2012 | Vol 1 | No 1 © Ficus Publishers. This Open Access article is governed by the Creative Commons Attribution License (http:// creativecommons.org/licenses/by/2.0), which gives permission for unrestricted use, non- commercial, distribution, and reproduction in all medium, provided the original work is properly cited. Submit Your Manuscript www.ficuspublishers.com www.ficuspublishers.com/JRBC.php Journal of Research in Biochemistry An International Open Access Online Research Journal Authors: Ibegbulem CO 1 , Nwaogu LA 1 , Alisi CS 1 , Igwe CU 1 , Egbung GE 2 and Chikezie PC 3 . Institution: 1.Department of Biochemistry, Federal University of Technology, Owerri, Nigeria. 2. Department of Biochemistry, University of Calabar, Calabar, Nigeria. 3. Department of Biochemistry, Imo State University, Owerri, Nigeria. Corresponding author: Ibegbulem CO. Email: ibemog@yahoo.com. Phone No: +2348037239349. Web Address: http://ficuspublishers.com/ documents/BC0005.pdf. Dates: Received: 12 Mar 2012 Accepted: 13 May 2012 Published: 04 Jun 2012 Article Citation: Ibegbulem CO, Nwaogu LA, Alisi CS, Igwe CU, Egbung GE and Chikezie PC. Anti-aggregation effects of processed yellow and white Garri Aqueous filtrates on sickle cell Trait Haemoglobin. Journal of Research in Biochemistry (2012) 1: 047-052 An International Online Open Access Publication group Original Research Journal of Research in Biochemistry Journal of Research in Biochemistry