Posters / International Journal of Antimicrobial Agents 42S2 (2013) S41–S159 S101 Public health and community-acquired infections P184 Epidemiology and molecular characteristics of diarrheal viruses detected in children from Bangladesh and Turkey T. Matsumoto 1 *, M.T. Mitui 1 , G. Bozdayi 2 , S. Ahmed 3 , B. Dalgic 4 , L. Kabir 3 , I. Bostanci 5 , A. Nishizono 1 , K. Ahmed 6 . 1 Microbiology, Faculty of Medicine, Oita University, Yufu, Japan, 2 Medical Microbiology, Faculty of Medicine, Gazi University, Ankara, Turkey, 3 Pediatrics, Institute of Mother and Child Health, Dhaka, Bangladesh, 4 Pediatric Gastroenterology, Faculty of Medicine, Gazi University, 5 Pediatrics, Ministry of Health Ankara Training and Education Hospital, Ankara, Turkey, 6 Research Promotion Institute, Oita University, Yufu, Japan E-mail address : ahmed@oita-u.ac.jp Introduction: Viral pathogens are responsible for severe diarrheal diseases in children. Annually 1.5 billion episodes and 1.5–2.5 million deaths are estimated to occur among children aged < 5 years worldwide. In Asian countries, about 30 to 70% of the diarrhea-related hospitalizations are attributable to rotavirus infection. However, little is known about other viral agents and co-infection in children with diarrhea in these children. Objectives: The purpose of this study was to investigate the epi- demiology and molecular characteristics of human bocavirus (HBoV), astrovirus (HAstV), norovirus GI and GII, (NoV) and adenovirus (AdV) in children from Turkey and Bangladesh. Methods: The stool samples were collected from children less than 5 years with acute diarrhea who attended to referral hospitals in Turkey and Bangladesh. These samples were negative for rotavirus. Results: Among 150 samples collected in Turkey, 46% were single and 12% were mixed pathogens. In Bangladesh, among 138 samples, 21% samples contained single pathogen and 68% samples contained mixed pathogens. In Turkey, AdV was most dominant agent, as single pathogen and it was significantly prevalent than that in Bangladesh. In Bangladesh, HoBV was the most common, as well as the high rate of co-infection with NoV GII and AdV. In Bangladesh, HBoV, NoV GII and AdV were most prevalent as mixed infection. Conclusion: Compared with Turkey in Bangladesh, enteric viruses were more prevalent and prone to be detected as multiple infec- tions. The epidemiology and molecular characteristics of circulating pathogens within population will lead to reducing the risks of unforeseen outbreak. P185 Highly virulent MLB1 astrovirus in Bhutanese children with diarrhea T. Yahiro 1 *, S. Wangchuk 2 , K.P. Tshering 3 , P. Bandhari 4 , S. Zangmo 2 , T. Dorji 5 , T. Matsumoto 1 , M.T. Mitui 1 , A. Nishizono 1 , K. Ahmed 6 . 1 Microbiology, Faculty of Medicine, Oita University, Yufu, Japan, 2 Public Health Laboratory, Department of Public Health, Ministry of Health, 3 Pediatrics, Jigme Dorji Wangchuk National Referral Hospital, Thimphu, 4 Pediatrics, Mongar Regional Referral Hospital, Mongar, 5 Communicable Disease Division, Department of Public Health, Ministry of Health, Thimphu, Bhutan, 6 Research Promotion Institute, Oita University, Yufu, Japan E-mail address : ahmed@oita-u.ac.jp Introduction: Human astrovirus (HuAstV) causes severe diarrhea in all age group worldwide, particularly in children under-2 year old, elderly persons, or otherwise immunocompromised persons are mostly affected and are at risk. AstVs are genetically closely related and infects a variety of animals, therefore is a public health concern for zoonosis. HAstV is classified into 8 genotypes. MLB1 is a new type of astrovirus identified in an Australian child with severe diarrhea. It has been also reported from Hong Kong, China, USA and Nigeria. Bhutan is a small landlocked country between China and India. Diarrhea is one of the important cause of childhood infections in Bhutan. However the contribution of different viruses in diarrhea is not clearly known. Furthermore due to abundant nature, people are in close association with different animals and are at risk of acquiring zoonotic infections. Objectives: This study was performed to know the prevalence and molecular characterization of AstVs from Bhutanese children with diarrhea. Methods: A total of 136 fecal samples were collected between February 2009 and February 2010 from children under-5 year old (average 10.6 month old, male-female ratio was 1: 1.09) with diarrhea attending at referral hospitals in Thimphu, Mongar and Gelephu. RT-PCR was performed to detect AstV in stool samples and nucleotide sequences of amplicons were determined. Phylogenetic analysis was done by neighbor joining (NJ) method using amino acid sequences of ORF1a (787 aa), ORF1b (506 aa) or ORF2 (756 aa). Branching pattern was evaluated by bootstrap analysis of 1000 replicates. Results: AstV was determined in 6 of 136 samples. Four were identified as type 1, clustered with strains prevalent in Thailand and India. MLB1 was detected in two (1.4%) children of one and 16 month old. Whole genome sequences of MLB1 strains showed that ORF1a, ORF1b or ORF2 of Bhutanese strains had 98–99% identity with strains reported from Nigeria, Hong Kong and China. Conclusion: Among the diarrhea caused by AstVs in Bhutan, MLB1 was responsible for a significant proportion of diarrhea in children compared with other countries. All affected children were under-2 year old. In several countries MLB1 has not been detected or a rare cause of diarrhea. Further surveillance is necessary to understand the epidemiology and evolution of MLB1 in Bhutan. P186 New self-administering tool for assessing urinary symptoms and quality of life in female patients with acute uncomplicated cystitis J.F. Alidjanov 1 *, U.A. Abdufattaev 1 , F.A. Akilov 1 , K.G. Naber 2 , F.M. Wagenlehner 3 . 1 Republican Specialized Center of Urology, Tashkent, Uzbekistan, 2 Technical University of Munich, Munich, 3 Justus-Liebig University, Clinic For Urology, Pediatric Urology and Andrology, Giessen, Germany E-mail address : dr.alidjanov@gmail.com Introduction: Symptoms of lower urinary tract infections (UTI) in women are heterogenous and bacterial infection is not always the cause of the symptoms. Objectives: The aim of our study was therefore to develop a reliable and valid self-administered questionnaire to assess acute uncomplicated cystitis (AUC) in Uzbek and Russian speaking women with high rates of specificity and sensitivity. Methods: Developed urinary symptoms and quality of life tool (USQOLAT) is self-administered questionnaire in Russian and Uzbek languages and contains questions about 6 typical symptoms of AUC in women, ranged from most common to less. It includes additional 4 questions asking about other symptoms which could help to differentiate AUC from other diseases having similar symptoms and a quality of life domain. This questionnaire was initially developed in Russian and Uzbek languages. Pilot and clinical tests of Uzbek and Russian versions of the tool were performed in 20 women with confirmed AUC. These women were then interviewed for revealing disadvantages of tool’s appearanceand design. After performing corrective action based on feedback, the final questionnaire was filled by 213 women with mean age 32 (ranged from 15 to 73), visiting urologist’s office. Results: Sensitivity and specificity for Russian version of USQOLAT were 95.0% and 82.6% correspondingly; Calculated test accuracy of the Russian version of USQOLAT was 88.4%, power was 95%; Reliability of Russian version of the tool was also good (Cronbach’s alpha of 0.85, split-half reliability and Guttman split-half – 0.92, correlation between first and second half – 0.86). Similar results were obtained from analysis of Uzbek version of the test as follows: Sensitivity and specificity – 92.7% and 86.3% respectively; Test accuracy and power of the test were 89.3% and 92% correspondingly; Reliability was also good (Cronbach’s alpha of 0.85, split-half reliability and Guttman split-half – 0.9, correlation between first and second half – 0.82). Calculated sensitivity to change was significant: T level – 16.5 with Z-level of 5.8 at p < 0.001. Scores of the test between main and control groups had statistically significant differences: Mean±SD 10.6±3.4 vs. 2.4±2.8 at p-level < 0.001 for main and control groups respectively.